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Rabbit Polyclonal TRPM5 Antibody

目录号: PC-78619

TRPM5 is a member of the TRPM (melastatin-like) subfamily which are Ca(2+)-permeable cation channels localized predominantly to the plasma membrane . TRPM5 is implicated in enhancing TRPA1 expression and may be involved in regulating insulin secretion. It has been reported that TRPM5 is N-linked glycosylated at a unique site and TRPM5 glycosylation seems not to be involved in channel trafficking, but mainly in its functional regulation.

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规格 价格 库存
50ul 1800.00 现货
100ul 3200.00 现货

基本信息

名称:兔多克隆T1R3抗体

描述:TRPM5 is a member of the TRPM (melastatin-like) subfamily which are Ca(2+)-permeable cation channels localized predominantly to the plasma membrane . TRPM5 is implicated in enhancing TRPA1 expression and may be involved in regulating insulin secretion. It has been reported that TRPM5 is N-linked glycosylated at a unique site and TRPM5 glycosylation seems not to be involved in channel trafficking, but mainly in its functional regulation.

克隆:多克隆

纯化方式:Antigen affinity purification.

宿主:Rabbit/IgG

浓度:2ug/ul

别名:

MLSN1 and TRP-related antibody

MLSN1- and TRP-related gene 1 protein antibody

MTR1 antibody

Novel protein similar to vertebrate transient receptor potential cation channel

subfamily M

member 5 antibody

Transient receptor potential cation channel subfamily M member 5 antibody

Transient receptor potential cation channel subfamily M member 5 antibody

Trpm5 antibody

TRPM5 transient receptor potential cation channel

subfamily M

member 5 antibody

TRPM5_HUMAN antibody

种属反应性:Human,Mouse,Rat

经测试应用:WB,IHC-P/IHC-Fr,IF/ICC

预测分子量:~131kDa

Swiss-Prot :Q9NZQ8

抗原:Synthetic peptide corresponding to a region derived from internal residues of human transient receptor potential cation channel, subfamily M, member 5.

应用:  WB:1:500-1000     IHC-P/IHC-Fr:1:50-200   IF:1:50-100  

存储形式:Liquid

存储液:PBS with 0.02% sodium azide, 50% glycerol, 0.5% BSA

储存温度:-20℃

 

 

Gel: 10%SDS-PAGE

Lysates (from left to right): Mouse heart tissue 

Amount of lysate: 40ug per lane

Primary antibody: 1/1200 dilution

Secondary antibody dilution: 1/8000(PC-80001)

Detection: Western Quick HRP substrate.(PC-90002)

Exposure time: 20 seconds

 

 

 

 

 

 

 

 

 

 

 

Western blot analysis of extracts of various cell lines, using TRPM5 antibody at 1:3000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG at 1:10000 dilution(PC-80001).
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: Western Quick HRP substrate.(PC-90002)
Exposure time: 90s.

 

 

 

 

 

 

 

 

 

 

 

 

Immunohistochemical analysis of paraffin-embedded Human gastric cancer tissue using at dilution 1/60.

 

存储温度 -20℃

相关说明文档

储备液配置

注:请根据所选浓度进行配置储备液,配置好储备液后进行分装低温保存,避免反复冻融!PCM提供多种溶剂,如:DMSO(PC-57930);玉米油(PC-86094);棉籽油(PC-30875)等

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