名称：兔多克隆mTOR (Ser2448)抗体

描述：The phosphatidylinositol kinase (PIK) family members fall into two distinct subgroups. The first subgroup contains proteins such as the PI 3- and PI 4-kinases and the second group comprises the PIK-related kinases. The PIK-related kinases include Atm, DNA-PKCS and FRAP. These proteins have in common a region of homology at their carboxy-termini that is not present in the PI 3- and PI 4-kinases. The Atm gene is mutated in the autosomal recessive disorder ataxia telangiectasia (AT) that is characterized by cerebellar degeneration (ataxia) and the appearance of dilated blood vessels (telangiectases) in the conjunctivae of the eyes. 

克隆：多克隆

纯化方式：Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy using non-phosphopeptide.

同种型：IgG

宿主：Rabbit

种属反应性：Human，Rat，Mouse

经测试应用：WB

预测分子量：250-289KDa

特异性：The antibody detects endogenous level of mTOR only when phosphorylated at serine 2448.

免疫原：Peptide sequence around phosphorylation site of serine 2448 (T-D-S(p)-Y-S) derived from Human mTOR.

浓度：WB：1:500-3000   

别称：FRAP; FRAP1; FRAP2; RAFT1;

存储液：Preservative: 0.03% Proclin 300，Constituents: 50% Glycerol, 0.01M PBS, PH 7.4

Western blot analysis of Phospho-mTOR (S2448) on different lysates with Phospho-mTOR (S2448) antibody at 1/1,000 dilution.
Lane 1: NIH/3T3 cell lysate treated with serum-starvation overnight
Lane 2: NIH/3T3 cell lysate treated with Insulin (100nM) for 10 minutes after serum-starvation overnight
Lysates/proteins at 10 µg/Lane.
Predicted band size: 288 kDa

Observed band size: 255 kDa
Exposure time: 2 minutes;

10% SDS-PAGE gel.