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Goat Polyclonal FOX3/Neun Antibody

  (neu-n)
目录号: PC-78001

Neun几乎存在于所有有丝分裂后中枢和外周神经元的细胞核中,并广泛应用于一般神经元的标记物。

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规格 价格 库存
50ug/50ul 3500.00 询问
100ug/100ul 5500.00 询问

基本信息

名称:Neu-N/Fox 3

描述:Neun几乎存在于所有有丝分裂后中枢和外周神经元的细胞核中,并广泛应用于一般神经元的标记物。Neu-N/Fox 3相对均匀地染色神经元细胞核。

克隆:多克隆

浓度:1mg/ml

纯化方式:Affinity purified antibody at 1mg/mL in 50% PBS, 50% glycerol plus 5mM NaN3

宿主:Goat

种属反应性:Human,Mouse,Rat

经测试应用:WB,IF/ICC,IHC-P,IHC-Fr

预测分子量:34 kDa(观测:46kDa)

抗原:N-terminal 100 amino acids of human Fox3 expressed in and purified from E. coli

浓度:WB:1:1000-2000  IF/ICC:1:500-3000   IHC-P/IHC-Fr:1:500-3000

存储液:PBS  PH7.2

储存温度:短时间2-8℃(1周左右),长期保存-20℃(1周以上)

Western blot of whole brain lysates using Goat Polyclonal NeuN antibody(PC-78001), dilution 1:1,000 in green:

[1] protein marker (red); [2] mouse brain; [3] rat brain. Bands at 46k and 48 kDa correspond to protein isotypes of FOX3/NeuN.

Immunofluorescent analysis of a section of adult rat hippocampus stained with Goat Polyclonal NeuN antibody(PC-78001), dilution 1:2,000 in red,MAP2  in green. Nuclear DNA was revealed in blue using the DAPI stain. Following transcardial perfusion of mouse with 4% paraformaldehyde, brain was post fixed for 24 hours, cut to 45μM, and free-floating sections were stained with the above antibodies. The Fox3/NeuN antibody stains the nuclei of neurons in the hippocampus while the MAP2 antibody stains the dendritic processes of neurons.

Chromogenic immunostaining of a formalin fixed paraffin embedded rat hippocampus section with Goat Polyclonal NeuN antibody(PC-78001), dilution 1:2,000, detected in DAB (brown) following the ABC method. Hematoxylin (blue) was used as the counterstain. The FOX3/NeuN antibody labels the nuclei of neurons in the hippocampus.

存储温度 -20℃

相关说明文档

储备液配置

注:请根据所选浓度进行配置储备液,配置好储备液后进行分装低温保存,避免反复冻融!PCM提供多种溶剂,如:DMSO(PC-57930);玉米油(PC-86094);棉籽油(PC-30875)等

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