名称：Glial Fibrillary Acidic Protein (GFAP)

描述：GFAP是胶质纤维酸性蛋白（glial fibrillary acidic protein）的英文简称，是星形胶质细胞活化的标志物。GFAP是一种III型中间丝状蛋白，参与星形胶质细胞的构成。在胚胎和胎儿期，中枢神经系统的放射状胶质细胞也表达GFAP。

克隆：多克隆

浓度：1mg/ml

纯化方式：Affinity purified antibody at 1mg/mL in 50% PBS, 50% glycerol plus 5mM NaN3

宿主：Goat

种属反应性：Human，Mouse，Rat

经测试应用：WB，IF/ICC，IHC-P，IHC-Fr

预测分子量：50 kDa

抗原：Recombinant full length human GFAP isotype 1 expressed in and purified from E. coli.

浓度：WB：1:1000-5000  IF/ICC：1:500-3000   IHC-P/IHC-Fr：1:500-2000

存储液：PBS  PH7.2

储存温度：短时间2-8℃（1周左右），长期保存-20℃（1周以上）

Western blot  of brain lysates from different species using Goat Polyclonal GFAP antibody（PC-70001）, dilution 1:5,000 in green:

[1] protein standard (red)； [2] rat cortex； [3] rat cerebellum,；[4] mouse cortex； [5] mouse cerebellum,；[6] cow cortex,； [7] cow cerebellum.

Immunofluorescent analysis of mouse hippocampus section stained with Goat Ployclonal GFAP antibody，dilution 1:5,000 in green，Neun dilution 1:2,000 in red.Following transcardial perfusion of mouse with 4% paraformaldehyde, brain was post fixed for 24 hours, cut to 45μM, and free-floating sections were stained with above antibodies. The GFAP antibody stains the network of astrocytic glial cells, while the NeuN antibody specifically labels nuclei and proximal perikarya of neurons.

Chromogenic immunostaining of a formalin fixed paraffin embedded mouse cerebral cortex section with Goat Ployclonal GFAP antibody,

dilution 1:5,000 ,detected in DAB (brown) following the ABC method. Hematoxylin (blue) was used as the counterstain. In normal brain tissue, the GFAP antibody specifically labels astrocytes.