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Chicken Polyclonal MAP2 Antibody

目录号: PC-70002

MAP 2是一个神经元特异性的细胞骨架蛋白,可以作为神经细胞的表型的标记物。

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规格 价格 库存
25ul 4500.00 询问
50ul 8500.00 询问

基本信息

名称:MAP2(Microtubule associated protein 2)

描述:MAP 2是一个神经元特异性的细胞骨架蛋白,可以作为神经细胞的表型的标记物。MAP-2在脊椎动物胚胎和成年神经系统的组织中表达,在神经前体中表达较弱,但在随后显著提高。

克隆:多克隆

纯化方式:Concentrated IgY preparation plus 5mM NaN3

同种型:IGY

宿主:Chicken

种属反应性:Human,Mouse,Rat

经测试应用:WB,IF/ICC,IHC-P,IHC-Fr

预测分子量:200 kDa

抗原:Mix of recombinant human constructs of projection domain sequences, amino acids 235-1588

浓度:WB:1:1000-2000  IF/ICC:1:500-1000   IHC-P/IHC-Fr:1:200-800

存储液:PBS  PH7.2

储存温度:2-8℃,请勿冻存

使用鸡多克隆的MAP-2抗体进行western blot测试,1:50000稀释,泳道1为标准marker;泳道2为成年大鼠脑;泳道3为胚胎大鼠脑;泳道4为成年小鼠脑(280kDa处的条带对应的是MAP2蛋白的两种主要亚型MAP2A和MAP2B,如果在高浓度下,该抗体也可以检测到同种型的较小片段,如约70kDa处)

Immunofluorescent analysis of cortical neuron-glial cell culture from E20 rat stained with chicken Polyclonal MAP2 Antibody,dilution 1:10,000 in red,mouse monoclonal Tau antibody dilution 1:2,000 in green,The blue is DAPI staining of nuclear DNA.Overlap of MAP2 and Tau staining results in an orange-yellow color.

Chromogenic Immunostaining of a formalin fixed paraffin embedded rat cerebellum section with Chicken Polyclonal MAP2 Antibody dilution 1:10,000,detected in DAB (brown) following the ABC method. Hematoxylin (blue) was used as the counterstain.

 

 

 

 

存储温度 2-8℃

相关说明文档

储备液配置

注:请根据所选浓度进行配置储备液,配置好储备液后进行分装低温保存,避免反复冻融!PCM提供多种溶剂,如:DMSO(PC-57930);玉米油(PC-86094);棉籽油(PC-30875)等

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