名称：兔单克隆Sirt3n抗体

描述：NAD-dependent deacetylase sirtuin-3, mitochondrial also known as SIRT3 is a protein that in humans is encoded by the SIRT3 gene. SIRT3 is member of the mammalian sirtuin family of proteins, which are homologs to the yeast Sir2 protein. SIRT3 exhibits NAD+-dependent deacetylase activity. Members of the sirtuin family are characterized by a sirtuin core domain and grouped into four classes, and the protein encoded by this gene is included in class I of the sirtuin family. The human sirtuins have a range of molecular functions and have emerged as important proteins in aging, stress resistance, and metabolic regulation. Yeast sirtuin proteins are known to regulate epigenetic gene silencing and suppress recombination of rDNA. In addition to protein deacetylation, studies have shown that the human sirtuins may also function as intracellular regulatory proteins with mono ADP ribosyltransferase activity.

克隆：单克隆

别称：

hSIRT 3 antibody

hSIRT3 antibody

Mitochondrial nicotinamide adenine dinucleotide dependent deacetylase antibody

NAD dependent deacetylase sirtuin 3 mitochondrial antibody

NAD-dependent protein deacetylase sirtuin-3, mitochondrial antibody

Regulatory protein SIR2 homolog 3 antibody

Silent mating type information regulation 2 S.cerevisiae homolog 3 antibody

Sir 2 like 3 antibody

SIR 2 like protein 3 antibody

SIR 3 antibody

SIR2 L3 antibody

Sir2 like 3 antibody

SIR2 like protein 3 antibody

SIR2-like protein 3 antibody

SIR2L3 antibody

SIR3_HUMAN antibody

SIRT 3 antibody

SIRT3 antibody

Sirtuin 3 antibody

Sirtuin silent mating type information regulation 2 homolog 3 (S. cerevisiae) antibody

Sirtuin type 3 antibody

Sirtuin3 antibody

纯化方式：Protein A affinity purified.

宿主：Rabbit/IgG

种属反应性：Human，Mouse，Rat

经测试应用：WB，IHC-P，IHC-Fr，IF/ICC，IP，ChIP

浓度：1mg/ml

分子量：44kDa

Uniprot No.：SwissProt: Q9NTG7 Human          SwissProt: Q8R104 Mouse     Entrez Gene: 293615 Rat   

抗原：Recombinant protein within human SIRT3 aa 51-399 / 399.

阳性对照：PANC-1 cell lysate, HepG2 cell lysate, A549 cell lysate, SiHa cell lysate, mouse liver tissue lysate, mouse kidney tissue lysate, rat liver tissue lysate, human liver tissue, human colon tissue, HeLa cell lysate, HEK-293 cell lysate, SW480 cell lysate, HT-29 cell lysate, rat kidney tissue lysate, human kidney tissue, mouse liver tissue, rat liver tissue, HepG2, NIH/3T3.

应用：  WB：1:500-1000   IHC-P/IHC-Fr：1:100-500    IF/ICC： 1:100-200   IP：1：500-1000     ChIP：Use 0.5~2 μg for 25 μg of chromatin.

存储形式：Liquid

存储液：TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

储存温度：-20℃

Western blot analysis of SIRT3 on different lysates with Rabbit Monoclonal Sirt3 Antibody at 1/2,000 dilution.

Lane 1: HeLa cell lysate (20 µg/Lane)
Lane 2: HEK-293 cell lysate (20 µg/Lane)
Lane 3: PANC-1 cell lysate (20 µg/Lane)
Lane 4: HepG2 cell lysate (20 µg/Lane)
Lane 5: SW480 cell lysate (20 µg/Lane)
Lane 6: A549 cell lysate (20 µg/Lane)
Lane 7: HT-29 cell lysate (20 µg/Lane)
Lane 8: SiHa cell lysate (20 µg/Lane)
Lane 9: Mouse liver tissue lysate (40 µg/Lane)
Lane 10: Mouse kidney tissue lysate (40 µg/Lane)
Lane 11: Rat liver tissue lysate (40 µg/Lane)
Lane 12: Rat kidney tissue lysate (40 µg/Lane)

Predicted band size: 44 kDa
Observed band size: 28 kDa

Immunocytochemistry analysis of HepG2 cells labeling SIRT3 with Rabbit Monoclonal Sirt3 Antibody at 1/100 dilution.

Immunohistochemical analysis of paraffin-embedded human kidney tissue with Rabbit Monoclonal Sirt3 Antibody at 1/1,000 dilution.

SIRT3 was immunoprecipitated from 0.2 mg HepG2 cell lysate with Rabbit Monoclonal Sirt3 Antibody  at 2 µg/25 µl agarose. Western blot was performed from the immunoprecipitate using Rabbit Monoclonal Sirt3 Antibody  at 1/2,000 dilution. Anti-Rabbit IgG for IP Nano-secondary antibody at 1/5,000 dilution was used for 1 hour at room temperature.

Lane 1: HepG2 cell lysate (input)
Lane 2: Rabbit Monoclonal Sirt3 Antibody  IP in HepG2 cell lysate
Lane 3: Rabbit IgG instead of Rabbit Monoclonal Sirt3 Antibody in HepG2 cell lysate