名称：山羊多克隆SOX9抗体

描述：SOX9 belongs to the SOX (SRY-like HMG box) family of transcription factors with diverse roles in development. SOX9 is expressed in mesenchymal progenitors that give rise to chondrocytes and osteoblasts. It is also expressed in the central nervous system, neural crest, intestine, pancreas, and testis. Mutations in SOX9 are associated with defects in sex determination, cartilage and bone development, as well as abnormalities of the heart, kidneys, brain, gut, and pancreas.

克隆：多克隆

纯化方式：Antigen Affinity-purified

宿主：Goat/IgG

免疫原：E. coli-derived recombinant human SOX9

特异性：Detects human SOX9 in direct ELISAs and Western blots. In direct ELISAs, approximately 25% cross-reactivity with recombinant human SOX10 is observed.

经测试应用：WB，IHC-P，IHC-Fr，ICC/IF

预测分子量：75kDa

应用：  WB：1:100-500     IHC-P/IHC-Fr：1:50-200       ICC/IF：1:50-200    

存储形式：Liquid

存储液：PBS（PH7.2）

储存温度：-20℃

Western blot shows lysates of HeLa human cervical epithelial carcinoma cell line, KATO-III human gastric carcinoma cell line, COLO 205 human colorectal adenocarcinoma cell line, and Hep3B human hepatocellular carcinoma cell line. PVDF membrane was probed with 0.5 µg/mL of Goat polyclonal SOX9 Antibody followed by HRP-conjugated Anti-Goat IgG Secondary Antibody .

SOX9 was detected in immersion fixed U251‑MG human malignant glioblastoma cell line (Positive) and absent in HEL 92.1.7 human erythroleukemic cell line (Negative) using Goat polyclonal SOX9 Antibody at 1.7 µg/ml for 3 hours at room temperature. Cells were stained using the  AF557-conjugated Anti-Goat IgG Secondary Antibody (red) and counterstained with DAPI (blue).