名称：小鼠单克隆LC3抗体

描述：Macroautophagy mediates the bulk degradation of cytoplasmic components. These components are delivered to lysosomes via autophagosomes. The rat microtubule-associated protein 1 light chain 3 (LC3), a homologue of yeast Atg8 (Aut7/Apg8), localizes to autophagosomal membranes after post-translational modifications. The C-terminal fragment of LC3 is cleaved immediately following synthesis to yield a cytosolic form called LC3-I. A subpopulation of LC3-I is further converted to an autophagosome-associating form, LC3-II. This antibody can detect both forms of LC3.

克隆：单克隆  4E12

浓度： 2mg/ml

同种型：IgG1 ĸ

宿主：Mouse

种属反应性：Human，Mouse，Rat，Hamster

经测试应用：WB，IP，IF/ICC，IHC-P，IHC-Fr，FC

抗原：Recombinant Human LC3 (MAP1LC3B: 1-120 a.a.)

形式：Liquid

浓度：WB：1:500-1000   IP：1:100-300  IF/ICC：1:50-200   IHC-P/IHC-Fr：1：50-200  FC：1:50-200

存储液：PBS containing 50% glycerol, pH 7.2. No preservative is contained.

储存温度：-20℃,避免污染

使用小鼠单克隆LC3抗体进行WB测试，泳道1：Hela；泳道2：NIH/3T3；泳道3：MEF；泳道4：PC12；泳道5：CHO；稀释比：1:500

使用小鼠单克隆LC3抗体进行免疫荧光测试，fixed starved MEF (a),nutrient MEF(b),starved NRK(normal rat kidney cell line)(c) and nutrient NRK(d) 

使用小鼠单克隆LC3抗体进行IP测试，泳道1和泳道2样品为Hela；泳道3和泳道4样品为NIH/3T3；泳道1和泳道3为同型对照。