名称：小鼠单克隆TWIST 1抗体

描述：Members of the myogenic determination family are basic helix-loop-helix (bHLH) proteins that can be separated into two classes. Class A proteins include the ubiquitously expressed E-box binding factors E12/E47, ITF2 and HEB (BETA1 or HTF4). Class B proteins such as MyoD, myogenin and NeuroD (BETA2) are transiently expressed and exhibit a much more limited tissue distribution. Class A proteins heterodimerize with class B proteins to activate DNA transcription. Working in opposition to these positively acting factors are a specialized group of proteins that function as dominant negative regulators. The novel basic helix-loop-helix (bHLH) transcription factor twist is a putative regulator of mesodermal differentiation and myogenesis. Twist is expressed throughout the epithelial somite but not in the myotome.

克隆：单克隆

浓度： 200ug/100ul

同种型：IgG1

宿主：Mouse

种属反应性：Human，Mouse

经测试应用：WB，IF/ICC，IHC-P，FC

预测分子量：25kDa

抗原：Recombinant protein

阳性对照：Human TWIST1 recombinant protein, TWIST1-hIgGFc transfected HEK293 cell lysate, NIH/3T3, JURKAT, HELA, A549, RAJI, OCM-1, Hela, cervical cancer tissue, colon cancer tissue.

形式：Liquid

浓度：WB：1:500-1000  IF/ICC：1:50-200   IHC-P：1：100-500  FC：1:100-200

存储液：Purified antibody in PBS with 0.05% sodium azide.

储存温度：-20℃,避免污染

Western blot analysis of TWIST1 on HEK293 (1) and TWIST1-hIgGFc transfected HEK293 (2) cell lysate using anti-TWIST1 antibody at 1/1,000 dilution.

Western blot analysis of TWIST1 on human TWIST1 recombinant protein using anti-TWIST1 antibody at 1/1,000 dilution.

Immunohistochemical analysis of paraffin-embedded cervical cancer tissue using anti-TWIST1 antibody. Counter stained with hematoxylin.

Flow cytometric analysis of Hela cells with TWIST1 antibody at 1/100 dilution (green) compared with an unlabelled control (cells without incubation with primary antibody; red).

ICC/IF staining TWIST1 (green) in Hela cells. The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.