名称：Green Fluorescent Protein (GFP)

描述：绿色荧光蛋白(Green fluorescent protein，简称GFP)，是由约238个氨基酸组成的蛋白质，从蓝光到紫外线都能使其激发，发出绿色荧光。

克隆：多克隆

浓度：1-1.1mg/ml

免疫原类型：重组蛋白

纯化方式：Affinity purified antibody at 1mg/mL in 50% PBS, 50% glycerol plus 5mM NaN3

同种型：IGG

宿主：Goat

种属反应性：All

经测试应用：WB，IP，ELISA，IF/ICC，IHC-P，IHC-Fr

预测分子量：27 kDa

抗原：The immunogen is a Green Fluorescent Protein (GFP) fusion protein corresponding to the full length amino acid sequence (246aa) derived from the jellyfish Aequorea victoria.

浓度：WB：1:200-2000   IF/ICC：1:500-1000    IHC-P/IHC-Fr：1:200-1000  IP：Use at an assay dependent concentration.

存储液：0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

储存温度：短时间2-8℃（1周左右），长期保存-20℃（1周以上）,避免污染

Sf-1+ neurons and their processes of the ventromedial nucleus of the hypothalamus in Mus musculus (coronal view, 20X magnification).4%PFA，1:500 dilution，Sections were counterstained with DAPI.

PCM Goat polyclonal anti-GFP antibody at a 1:1,000 dilution detects tau-GFP in cell bodies (large arrowhead) and axons of motorneurons (arrow) and interneurons (small arrowhead) in Drosophila melanogaster late stage embryonic central nervous system. Fluorochrome conjugated anti-Goat secondary antibody was used for detection at 1:300. Panel A shows a lateral view (ventral left) and Panels B and C show ventral views of whole mount embryos at 63x magnification (plus 2x digital zoom).

使用山羊多克隆GFP抗体进行WB测试1ug/ml，泳道1：HEK293裂解物，10ug；泳道2：HeLa裂解物，10ug；泳道3：CHO/K1 Lysate，10ug；泳道4：MDA-MB-231 (Human breast adenocarcinoma cell line) lysate，10ug；泳道5：A431 (Human epidermoid carcinoma cell line) lysate，10ug；泳道6：Jurkat Lysate，10ug；泳道7：NIH/3T3 Lysate；泳道8：E-coli HCP Control，50ng；泳道9：FLAG Positive Control Lysate，10ug；泳道10：Red Fluorescent Protein，50ng；泳道11：Green Fluorescent Protein，50ng；泳道12：Glutathione-S-Transferase Protein，50ng；泳道13：Maltose Binding Protein，50ng

使用山羊多克隆GFP抗体进行Weatern Blot测试1ug/ml，泳道1：标准marker；泳道2：GFP/Human Serum，0.01/0.02ul；泳道3：Human Serum，0.02ul；泳道4：GFP/Mouse Serum，0.01/0.02ul；泳道5：Mouse Serum，0.02ul；泳道6：GFP/Rat Serum，0.01/0.02ul；泳道7：Rat Serum，0.02ul