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Rabbit Monoclonal ADFP / PLIN2 antibody

目录号: PC-78742

The protein encoded by this gene belongs to the perilipin family, members of which coat intracellular lipid storage droplets. This protein is associated with the lipid globule surface membrane material, and maybe involved in development and maintenance of adipose tissue. However, it is not restricted to adipocytes as previously thought, but is found in a wide range of cultured cell lines, including fibroblasts, endothelial and epithelial cells, and tissues, such as lactating mammary gland, adrenal cortex, Sertoli and Leydig cells, and hepatocytes in alcoholic liver cirrhosis, suggesting that it may serve as a marker of lipid accumulation in diverse cell types and diseases. Alternatively spliced transcript variants have been found for this gene.

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规格 价格 库存
50ul 1800.00 现货
100ul 3200.00 现货

基本信息

名称:兔单克隆Perilipin-2抗体

描述:The protein encoded by this gene belongs to the perilipin family, members of which coat intracellular lipid storage droplets. This protein is associated with the lipid globule surface membrane material, and maybe involved in development and maintenance of adipose tissue. However, it is not restricted to adipocytes as previously thought, but is found in a wide range of cultured cell lines, including fibroblasts, endothelial and epithelial cells, and tissues, such as lactating mammary gland, adrenal cortex, Sertoli and Leydig cells, and hepatocytes in alcoholic liver cirrhosis, suggesting that it may serve as a marker of lipid accumulation in diverse cell types and diseases. Alternatively spliced transcript variants have been found for this gene.

克隆:单克隆

别称:

ADFP antibody

Adipophilin antibody

Adipose differentiation related protein antibody

Adipose differentiation-related protein antibody

ADRP antibody

MGC10598 antibody

Perilipin-2 antibody

PLIN2 antibody

PLIN2_HUMAN antibody

纯化方式:Protein A affinity purified.

宿主:Rabbit/IgG

种属反应性:Human,Mouse,Rat

经测试应用:WB,IHC-P,IHC-Fr,IF/ICC

浓度:1mg/ml

预测分子量:Predicted band size: 48 kDa

抗原:Synthetic peptide within Human ADFP aa 18-67 / 437.

阳性对照:HepG2 cell lysate, JAR cell lysate, Human liver tissue lysate, HeLa, human liver tissue, human liver carcinoma tissue, human stomach tissue.

应用:  WB:1:500-1000       IHC-P/IHC-Fr:1:50-200        IF/ICC:1:50-200

亚细胞定位:Membrane.

存储形式:Liquid

存储液:1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

储存温度:-20℃

 

Western blot analysis of ADFP / PLIN2 on different lysates with Rabbit Monoclonal ADFP / PLIN2 antibody  at 1/1,000 dilution.
Lane 1: HepG2 cell lysate
Lane 2: JAR cell lysate
Lane 3: Human liver tissue lysate
Lysates/proteins at 40 µg/Lane.
Predicted band size: 48 kDa
Observed band size: 45 kDa
Exposure time: 1 minute 2 seconds; ECL: PC-90002
4-20% SDS-PAGE gel.

 

 

 

☑ Knockdown (KD)

Western blot analysis of ADFP / PLIN2 on different lysates with Rabbit Monoclonal ADFP / PLIN2 antibody 1/2,000 dilution.

Lane 1: HAP1-parental cell lysate
Lane 2: HAP1-ADFP / PLIN2 KD cell lysate

Lysates/proteins at 10 µg/Lane.
Predicted band size: 48 kDa
Observed band size: 48 kDa
Exposure time: 3 minutes; ECL: PC-90002
4-20% SDS-PAGE gel.

 

 

 

 

Immunocytochemistry analysis of HeLa cells labeling ADFP / PLIN2 with Rabbit Monoclonal ADFP / PLIN2 antibody at 1/50 dilution.

 

 

 

 

 

 

 

 

 

 

 

Immunohistochemical analysis of paraffin-embedded human liver carcinoma tissue with Rabbit Monoclonal ADFP / PLIN2 antibody at 1/100 dilution.

 

存储温度 -20℃

相关说明文档

储备液配置

注:请根据所选浓度进行配置储备液,配置好储备液后进行分装低温保存,避免反复冻融!PCM提供多种溶剂,如:DMSO(PC-57930);玉米油(PC-86094);棉籽油(PC-30875)等

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