名称：山羊多克隆CD31抗体

描述：PECAM-1 is found on the surface of platelets, monocytes, neutrophils, and some types of T-cells, and makes up a large portion of endothelial cell intercellular junctions. The encoded protein is a member of the immunoglobulin superfamily and is likely involved in leukocyte transmigration, angiogenesis, and integrin activation. CD31 is also expressed in certain tumors, including epithelioid hemangioendothelioma, epithelioid sarcoma-like hemangioendothelioma, other vascular tumors, histiocytic malignancies, and plasmacytomas. It is rarely found in some sarcomas, such as Kaposi's sarcoma and carcinomas. In immunohistochemistry, CD31 is used primarily to demonstrate the presence of endothelial cells in histological tissue sections. This can help to evaluate the degree of tumour angiogenesis, which can imply a rapidly growing tumor.

克隆：多克隆

纯化方式：Antigen Affinity-purified

宿主：Goat/IgG

免疫原：Mouse myeloma cell line NS0-derived recombinant mouse CD31/PECAM-1，Glu18-Lys590，Accession # Q08481

特异性：Detects human and mouse CD31/PECAM-1 in direct ELISAs and Western blots. In direct ELISAs and Western blots, approximately 10% cross-reactivity with recombinant porcine CD31 is observed.

经测试应用：WB，IHC-P，IHC-Fr，ICC/IF，FC

预测分子量：130kDa

应用：  WB：1:200-500     IHC-P/IHC-Fr：1:40-100       ICC/IF：1:40-100      FC：1:100-800

存储形式：Liquid

存储液：PBS（PH7.2）

储存温度：-20℃

Western blot shows lysates of bEnd.3 mouse endothelioma cell line. PVDF membrane was probed with 0.5 µg/mL of Goat polyclonal CD31/PECAM-1 Antibody followed by HRP-conjugated Anti-Goat IgG Secondary Antibody

CD31/PECAM-1 was detected in immersion fixed frozen sections of mouse embryo (14 d.p.c.) using Goat polyclonal CD31/PECAM-1 Antibody at 10 µg/mL for 1 hour at room temperature followed by incubation with the Anti-Goat IgG HRP Polymer Antibody. Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue).

Immunohistochemistry (paraffin-embedded) of Native Adult Rat lung tissue stained for Abca3 (red), CD31/PECAM-1 (green), and Dclk1 (cyan). Image from a verified customer review.

CD31/PECAM-1 was detected in immersion fixed frozen sections of mouse embryo (E13.5) using Goat polyclonal CD31/PECAM-1 Antibody at 10 µg/mL overnight at 4 °C. Tissue was stained using the NorthernLights™ 557-conjugated Anti-Goat IgG Secondary Antibody (yellow) and counterstained with DAPI (blue).

Rat splenocytes were stained with Goat polyclonal CD31/PECAM-1 Antibody ( filled histogram) or isotype control antibody ( open histogram), followed by Allophycocyanin-conjugated Anti-Goat IgG Secondary Antibody