名称：兔多克隆cGAS抗体

描述：cGAS Antibody (C-1) is a high quality monoclonal cGAS antibody (also designated cGAS antibody) suitable for the detection of the cGAS protein of human origin. cGAS Antibody (C-1) is available as the non-conjugated anti-cGAS antibody. The presence of foreign DNA in the cytoplasm induces an antiviral host immune response. DNA in the cytoplasm triggers the production of interferons by activating and synthesis of second messenger cyclic guanosine monophosphate-adenosine monophosphate (cyclic GMP-AMP, or cGAMP). cGAS (cyclic GMP-AMP synthase), also known as MB21D1 (Mab-21 domain containing 1), h-cGAS or C6orf150, is a 522 amino acid cytoplasmic nucleotidyltransferase that catalyzes the formation of cyclic GMP-AMP (cGAMP) from ATP and GTP. cGAS is suggested to have antiviral activity by acting as a key cytosolic DNA sensor. cGAS binds to cytosolic DNA, which leads to cGAMP synthesis and activation of TMEM173, thereby trigger type-I interferon production. Expressed in monocytic cell line THP1, cGAS exists as two alternatively spliced isoforms and is encoded by a gene located on human chromosome 6q13.

克隆：多克隆

别称：C6orf150 antibody

cGAMP synthase antibody

cGAS antibody

CGAS_HUMAN antibody

cGMP Synthase antibody

Chromosome 6 open reading frame 150 antibody

Cyclic GMP-AMP synthase antibody

h-cGAS antibody

Hypothetical protein LOC115004 antibody

Mab 21 domain containing 1 antibody

Mab-21 domain-containing protein 1 antibody

MB21D1 antibody

MGC131892 antibody

MGC142166 antibody

MGC142168 antibody

OTTHUMP00000016743 antibody

OTTHUMP00000039330 antibody

protein MB21D1 antibody

Uncharacterized protein C6orf150 antibody

纯化方式：Protein A affinity purified.

宿主：Rabbit/IgG

种属反应性：Human，Mouse，Rat

经测试应用：WB，IHC-P，IF/ICC

预测分子量：59 kDa

RRID: AB_2770305

Uniprot No.：Q8N884 Human, Q8C6L5 Mouse

抗原：Recombinant fusion protein containing a sequence corresponding to amino acids 350-522 of human cGAS (NP_612450.2).

应用：  WB：1:500-1000   IHC-P：1:50-200    IF/ICC:  1:50-200

存储形式：Liquid

存储液：PBS with 0.09% Sodium azide,50% glycerol,pH7.3.

储存温度：-20℃

Western blot analysis of lysates from HeLa cells using Rabbit Polyclonal cGAS Antibody at 1/1000 dilution.
Secondary antibody: 辣根过氧化物酶标记山羊抗兔IgG（H+L）(PC-80001) at 1:20000 dilution.
Lysates/proteins: 25 μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: Western Quick HRP substrate (PC-90002).
Exposure time: 30s.

Western blot analysis of lysates from HT-29 cells using Rabbit Polyclonal cGAS Antibody at 1/1000 dilution incubated overnight at 4℃.
Secondary antibody: 辣根过氧化物酶标记山羊抗兔IgG（H+L）(PC-80001) at 1:20000 dilution.
Lysates/proteins: 25 μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: Western Quick HRP substrate (PC-90002).
Exposure time: 60s.

ICC/IF analysis of NIH/3T cells using Rabbit Polyclonal cGAS Antibody.
Orange : Primary antibody
Blue : DAPI
Dilution : 1:50

ICC/IF analysis of PC-12 cells using Rabbit Polyclonal cGAS Antibody.
Orange : Primary antibody
Blue : DAPI
Dilution : 1:50

IHC-P analysis of Human colon carcinoma tissue using Rabbit Polyclonal cGAS Antibody.

Antigen retrieval : high pressure antigen retrieval with 10 mM citrate buffer pH 6.
Dilution : 1:50

IHC-P analysis of Human colon tissue using Rabbit Polyclonal cGAS Antibody.
Antigen retrieval : high pressure antigen retrieval with 10 mM citrate buffer pH 6.
Dilution : 1:50