名称：兔单克隆PDGF beta抗体

描述：Platelet-derived growth factor (PDGF) is a mitogen for mesenchyme- and glia-derived cells. PDGF consists of two chains, A and B, which dimerize to form functionally distinct isoforms, PGDF-AA, PDGF-AB and PDGF-BB. These three isoforms bind with different affinities to two receptor types, PDGFR-α and –β, which are endowed with protein tyrosine kinase domains. PDGFR-α can bind to both A and B subunits of PDGF, while PDGFR-β can only bind the B subunit. Ligand binding promotes either homo- or heterodimerization of the PDGF receptors in a specific manner. 

克隆：单克隆

别称：Beta platelet derived growth factor receptor antibody

Beta-type platelet-derived growth factor receptor antibody

CD 140B antibody

CD140 antigen-like family member B antibody

CD140b antibody

CD140b antigen antibody

IBGC4 antibody

IMF1 antibody

JTK12 antibody

OTTHUMP00000160528 antibody

PDGF R beta antibody

PDGF-R-beta antibody

PDGFR 1 antibody

PDGFR antibody

PDGFR beta antibody

PDGFR1 antibody

PDGFRB antibody

PGFRB_HUMAN antibody

Platelet derived growth factor receptor 1 antibody

Platelet derived growth factor receptor beta antibody

Platelet derived growth factor receptor beta polypeptide antibody

纯化方式：Protein A affinity purified.

宿主：Rabbit/IgG

种属反应性：Human，Mouse，Rat

经测试应用：WB，IHC-P，IP，IHC-Fr，IF/ICC，mIHC

浓度：1mg/ml

预测分子量：Predicted band size: 124 kDa

抗原：Recombinant protein within Human PDGFR beta aa 961-1,106 / 1,106.

阳性对照：SH-SY5Y cell lysate, C6 cell lysate, Mouse brain tissue lysate, Rat brain tissue lysate, SH-SY5Y, human spleen tissue, mouse brain tissue, mouse spleen tissue, rat spleen tissue.

应用：  WB：1:500-1000    IHC-P：1:50-200    IHC-Fr：1:50-200     IF/ICC：1:50-200    mIHC：1:500-2000

存储形式：Liquid

存储液：1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

储存温度：-20℃

Western blot analysis of PDGFR beta on different lysates with Rabbit anti-PDGFR beta antibody  at 1/1,000 dilution.
Lane 1: SH-SY5Y cell lysate (20 µg/Lane)
Lane 2: C6 cell lysate (20 µg/Lane)
Lane 3: Mouse brain tissue lysate (40 µg/Lane)
Lane 4: Rat brain tissue lysate (40 µg/Lane)
Predicted band size: 124 kDa
Observed band size: 190 kDa
Exposure time: 6 seconds; Western Quick HRP substrate: PC-90002;
4-20% SDS-PAGE gel.

Immunocytochemistry analysis of SH-SY5Y cells labeling PDGFR beta with Rabbit anti-PDGFR beta antibody at 1/100 dilution.

Immunofluorescence analysis of frozen mouse brain tissue with Rabbit anti-PDGFR beta antibody  at 1/200 dilution.

mIHC analysis of human tonsils tissue (Formalin/PFA-fixed paraffin-embedded sections) with Rabbit anti-PDGFR beta antibody  at 1/1,500 dilution. The immunostaining was performed with the TSA Kit . Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 30 mins at 95℃. DAPI (blue) was used as a nuclear counter stain. Image acquisition was performed with Olympus VS200 Slide Scanner.

mIHC analysis of mouse spleen tissue (Formalin/PFA-fixed paraffin-embedded sections) with Rabbit anti-PDGFR beta antibody at 1/2,000 dilution. The immunostaining was performed with TSA Kit. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 30 mins at 95℃. DAPI (blue) was used as a nuclear counter stain. Image acquisition was performed with Olympus VS200 Slide Scanner.

mIHC analysis of mouse kidney tissue (Formalin/PFA-fixed paraffin-embedded sections) with Rabbit anti-PDGFR beta antibody at 1/2,000 dilution. The immunostaining was performed with TSA Kit. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 30 mins at 95℃. DAPI (blue) was used as a nuclear counter stain. Image acquisition was performed with Olympus VS200 Slide Scanner.