名称：兔单克隆Histone H3 抗体

描述：In eukaryotes, DNA is wrapped around histone octamers to form the basic unit of chromatin structure. The octamer is composed of histones H2A, H2B, H3 and H4, and it associates with approximately 200 base pairs of DNA to form the nucleosome. The association of DNA with histones results in dense packing of chromatin, which restricts proteins involved in gene transcription from binding to DNA. 

克隆：单克隆

别称：H3 histone family, member A antibody     H3/A antibody     H31_HUMAN antibody        H3FA antibody

Hist1h3a antibody     HIST1H3B antibody     HIST1H3C antibody     HIST1H3D antibody     HIST1H3E antibody

HIST1H3F antibody   HIST1H3G antibody     HIST1H3H antibody     HIST1H3I antibody       HIST1H3J antibody

histone 1, H3a antibody    Histone cluster 1, H3a antibody     Histone H3.1 antibody      Histone H3/a antibody

Histone H3/b antibody     Histone H3/c antibody       Histone H3/d antibody      Histone H3/f antibody

Histone H3/h antibody    Histone H3/i antibody     Histone H3/j antibody     Histone H3/k antibody

Histone H3/l antibody

纯化方式：Protein A affinity purified.

宿主：Rabbit/IgG

种属反应性：Human，Mouse，Rat

经测试应用：WB，IHC-P，IHC-Fr，IF/ICC，ChIP，IP

浓度：1mg/ml

预测分子量：15kDa

抗原：Recombinant protein within human Histone H3 aa 85-136/136.

应用：  WB：1:500-5000   IHC-P/IHC-Fr：1:100-500     IF/ICC：1：50-200  ChIP：0.5-2ug for 25ug of chromatin   IP：1-2ug/sample

存储形式：Liquid

存储液：TBS (pH7.4), 0.05% BSA, 40% Glycerol，0.05%proclin300

储存温度：-20℃

Western blot analysis of Histone H3 on different lysates with Rabbit Monoclonal Histone H3 antibody at 1/20,000 dilution and competitor's antibody at 1/5,000 dilution.

  Lane 1: HeLa cell lysate
  Lane 2: A549 cell lysate
  Lane 3: HT-29 cell lysate
  Lane 4: HEK-293 cell lysate
  Lane 5: C2C12 cell lysate
  Lane 6: L-929 cell lysate
  Lane 7: C6 cell lysate
  Lysates/proteins at 10 µg/Lane.
  Predicted band size: 15 kDa

 Immunohistochemical analysis of paraffin-embedded human skin tissue with Rabbit anti-Histone H3 antibody at 1/5,000 dilution.

 Immunofluorescence analysis of paraffin-embedded rat testis tissue labeling Histone H3 with Rabbit  anti-Histone H3 antibody  at 1/100 dilution.

Histone H3 was immunoprecipitated in 0.2mg HeLa cell lysate with at 2 µg/25 µl agarose. Western blot was performed from the immunoprecipitate using Rabbit Monoclonal Histone H3 Antibody at 1/20,000 dilution. HRP标记山羊抗兔IgG重链二抗-消除轻链干扰 (PC-80955) at 1/5,000 dilution was used for 1 hour at room temperature.
Lane 1: HeLa cell lysate (input)
Lane 2: Rabbit Monoclonal Histone H3 Antibody  IP in HeLa cell lysate
Lane 3: Rabbit IgG instead of Rabbit Monoclonal Histone H3 Antibody  in HeLa cell lysate
Blocking/Dilution buffer: 5% NFDM/TBST
Exposure time: 1 minute 59 seconds; Western Quick HRP substrate（PC-90002）