名称：兔多克隆HERC5抗体

描述：HERC5 (E3 ISG15 - protein ligase HERC5) is a major E3 ligase for ISG15 conjugation. It acts a positive regulator of innate antiviral response in cells induced by interferon. HERC5 functions as a part of the ISGylation machinery that recognizes target protein in a broad and relatively non-specific manner. It catalyzes ISGylation of IRF3 - which results in sustained activation, it attenuates IRF3-PIN1 interaction - which antagonizes IFR3 ubiquitination and degradation, and boosts the antiviral repsonse. HERC5 is physically assocated with polyribosomes and broadly modifies newly synthesized proteins in a cotranslational manner.

克隆：多克隆

浓度： 1mg/ml

同种型：IgG

宿主：Rabbit

种属反应性：Human，Mouse，Rat

经测试应用：WB，IHC-P

分子量：117kDa

免疫原：A synthesized peptide derived from human HERC5(Accession Q9UII4), corresponding to amino acid residues G303-E353.

纯化方式：Affinity chromatography

别称：Cyclin-E-binding protein 1; E3 ISG15--protein ligase HERC5; HECT domain and RCC1-like domain-containing protein 5; hect domain and RLD 5; probable E3 ubiquitin-protein ligase HERC5; ubiquitin ligase; unnamed protein product

形式：Liquid

浓度：WB：1:500-2000       IHC-P：1:50-200  

存储液：PBS, pH 7.4, with 50% glycerol

储存温度：-20℃

Western blot analysis of HERC5 in HEK-293 cell lysate (left lane: treated with blocking peptide). Samples were incubated with HERC5 polyclonal antibody.

Immunohistochemistry analysis of HERC5 in rat testis tissue. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. Samples were incubated with HERC5 polyclonal antibody using a dilution of 1:100 (4°C overnight) followed by HRP conjugated anti-Rabbit secondary antibody.

Immunohistochemistry analysis of HERC5 in mouse testis tissue. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. Samples were incubated with HERC5 polyclonal antibody using a dilution of 1:100 (4°C overnight) followed by HRP conjugated anti-Rabbit secondary antibody.