名称：Green Fluorescent Protein (GFP)

描述：绿色荧光蛋白(Green fluorescent protein，简称GFP)，是由约238个氨基酸组成的蛋白质，从蓝光到紫外线都能使其激发，发出绿色荧光。

克隆：多克隆

浓度：1mg/ml

纯化方式：Affinity purified antibody at 1mg/mL in 50% PBS, 50% glycerol plus 5mM NaN3

同种型：IGG

宿主：Goat

种属反应性：All

经测试应用：WB，IF/ICC，IHC-P，IHC-Fr

预测分子量：27 kDa

抗原：Recombinant AcGFP protein purified from E. coli

浓度：WB：1:1000-3000  IF/ICC：1:500-2000   IHC-P/IHC-Fr：1:500-2000

存储液：PBS  PH7.2

储存温度：短时间2-8℃（1周左右），长期保存-20℃（1周以上）,避免污染

使用山羊多克隆GFP抗体进行HEK293细胞裂解物的测试，稀释比：1:1000，泳道1为标准marker；泳道2为HEK293对照组；泳道3为GFP转染的HEK293，泳道4为mCherry转染的HEK293（GFP只识别GFP的蛋白，不会识别到mCherry蛋白）

Above, mice transgenic for green fluorescent protein (GFP) under the Thy1 promoter express GFP in cerebral pyramidal cells. The image is of a 400μM maximum intensity optical section taken from an entire Thy1-GFP mouse brain “cleared” in the Lifecanvas lab. Lifecanvas scientists then stained the brain withgoat polyclonal GFP antibody coupled to a red dye. As expected, the green and red signals superimpose in the perikarya of the pyramidal cells which appear yellowish. The antibody also stains the apical dendrites which have a lower level of GFP expression, effectively amplifying the GFP signal.

Chromogenic immunostaining of formalin fixed paraffin embedded GFP transduced mouse brain with goat Polyclonal GFP antibody, dilution 1:5,000, detected in DAB (brown) using the ABC method. Hematoxylin (blue) was used as the counterstain.