名称：MAP2，MAP2A/B

描述：MAP 2是一个神经元特异性的细胞骨架蛋白，可以作为神经细胞的表型的标记物。MAP-2在脊椎动物胚胎和成年神经系统的组织中表达，在神经前体中表达较弱，但在随后显著提高。

克隆：多克隆

浓度：1mg/ml

纯化方式：Affinity purified antibody at 1mg/mL in 50% PBS, 50% glycerol plus 5mM NaN3

宿主：Goat

种属反应性：Human，Mouse，Rat

经测试应用：WB，IF/ICC，IHC-P，IHC-Fr

预测分子量：200 kDa（观测：280kDa）

抗原：Recombinant human projection domain sequences, amino acids 377-1505

浓度：WB：1:1000-2000  IF/ICC：1:500-3000   IHC-P/IHC-Fr：1:500-3000

存储液：PBS PH7.2

储存温度：短时间2-8℃（1周左右），长期保存-20℃（1周以上）

使用山羊多克隆的MAP-2抗体进行western blot测试，1:50000稀释，泳道1为标准marker；泳道2为大鼠全脑；泳道3为小鼠全脑；（280kDa处的条带对应的是MAP2蛋白的两种主要亚型MAP2A和MAP2B，如果在高浓度下，该抗体也可以检测到同种型的较小片段，如约70kDa处）

Immunofluorescent analysis of rat brain stem section stained with Goat Polyclonal MAP2 Antibody，dilution 1:2,000 in red，mouse monoclonal MBP antibody dilution 1:5,000 in green，Following transcardial perfusion of rat with 4% paraformaldehyde, brain was post fixed for 24 hours, cut to 45μM, and free-floating sections were stained with above antibodies. The Goat Polyclonal MAP2 antibody labels MAP2 protein in the perikarya and dendrites of the most neurons, notably motorneurons in the brain stem, and the MBP antibody stains myelin sheath around axons.

Chromogenic immunostaining of a formalin fixed paraffin embedded rat hippocampus section with Goat Polyclonal MAP2 Antibody,  dilution 1:2,000, detected in DAB (brown) following the ABC method. Hematoxylin (blue) was used as the counterstain.