名称：鸡单克隆PSD95抗体

描述：PSD-95 is a member of the MAGUK (membrane associated guanylate kinase) family (which also includes PSD-93, SAP97 and SAP102) and functions as a scaffold protein that can cluster membrane receptors, ion channels, including NMDA receptor subunits, Kv1 potassium channels and Caspr2. The protein is encoded by DLG4, contains hallmark PDZ, SH3 and GuK domains and is found at post synaptic densities where it plays an important role in the formation and maintenance of synaptic junctions. Indeed, overexpression or depletion of DLG4 changes the ratio of excitatory to inhibitory synapses in hippocampal neurons. Antibodies against PSD95/DLG4 are frequently used in conjunction with pre-synaptic markers to localize synapses by microscopy。

克隆：单克隆 K28/43

浓度： 1mg/mL

同种型：IgY

宿主：Chicken

种属反应性：Human，Mouse，Rat

经测试应用：WB，IF/ICC，IHC-P/IHC-Fr，IP

预测分子量：95~110kDa

抗原：Fusion protein amino acids 77-299 (PDZ domains 1 and 2) of human PSD-95 (accession number P78352) produced recombinantly in E. Coli

特异性：No cross-reactivity against recombinant Chapsyn-110, SAP97 or SAP102

别称：Disks large homolog 4 (Postsynaptic density protein 95) (PSD-95) (Synapse-associated protein 90) (SAP-90) (SAP90)

浓度：WB：1:100-1000  IF/ICC：1:50-500   IHC-P/IHC-Fr：1:50-500  

存储液：1X PBS, 0.05% Sodium Azide pH 7.4

储存温度：-20℃,避免污染

Representative image of 4% paraformaldehyde fixed free-floating mouse cerebellum sections stained with anti-PSD-95 recombinant chicken monoclonal antibody (red) at 1:1000 dilution and co-stained with anti-Calretinin rabbit polyclonal antibody (green).

Formalin fixed, paraffin-embedded rat brain cerebellum region showing staining of PSD-95. The sections were stained with anti-PSD-95 recombinant chicken monoclonal antibody (green) at 1:500 dilution.

Formalin fixed, paraffin-embedded rat brain cerebellum region showing staining of PSD-95. The sections were stained with anti-PSD-95 recombinant chicken monoclonal antibody (green) at 1:500 dilution.

Western blotting of rat brain homogenate with anti-PSD-95 mouse Mab (PC-78823) and anti-PSD-95 recombinant chicken Mab antibodies (2 µg/ml). The mouse Mab and chicken Mab were detected with anti-mouse-HRP and anti-chicken-HRP, respectively. Chicken anti-PSD-95 Mab recognizes endogenous PSD-95 with similar sensitivity as the anti-PSD-95 in the rat brain homogenate at ~95-110 kDa.