名称：小鼠单克隆VGLUT 1抗体

描述：Vesicular glutamate transporter 1 (Vglut1) is encoded by the gene Slc17a7 and belongs to the vesicular glutamate transporter family. Vglut1 is expressed in glutamatergic neurons in brain regions including the cerebellum, cerebral cortex and hippocampus at synaptic vesicles.

克隆：单克隆 N28/9

同种型：IgG1

宿主：Mouse

分子量：52 kDa

种属反应性：Human, Mouse, Rat

经测试应用：WB，IHC-P/IHC-Fr，IF/ICC，Flow

浓度：1mg/ml

特异性：Does not cross-react with VGlut2 or VGlut3

生产说明：Produced by in vitro bioreactor culture of hybridoma line followed by Protein A affinity chromatography. Purified mAbs are >90% specific antibody.

纯化方式：Purified by Protein A chromatography.

抗原：Fusion protein amino acids 493-560 (cytoplasmic C-terminus) of rat VGlut1 (accession number Q62634) produced recombinantly in E. Coli

浓度：WB：1:100-5000    IHC-P：1:100-500      IF/ICC：1:50-100

存储液：10 mM Tris, 50 mM Sodium Chloride, 0.065% Sodium Azide pH 7.4

储存温度：-20℃,避免污染

Coronal sections of rat cerebellum, stained with human patient autoantibodies (E, red) and anti-vGlut1 (F, green labeling, Cat no PC-76579, 1:300). Colocalization was not observed when mixing the anti-VGlut1 with the autoantibodies (merge G, red labeling + green labeling), In addition, autoantibody labeling was identified with Cy3 red labeling, while the antibodies were identified with Alexa488 green labeling.

Immunoblot against extracts of COS cells transfected with untagged VGlut or Kv2.1 plasmid