名称：小鼠单克隆CAMK II 抗体

描述：CaMKII is an important member of the calcium/calmodulin-activated protein kinase family, functioning in neural synaptic stimulation and T-cell receptor signaling. CaMKII is expressed in many different tissues but is specifically found in the neurons of the forebrain and its mRNA is found within the dendrites and the soma of the neuron. The CaMKII that is found in the neurons consist of two subunits of 52 (termed alpha genes) and 60 kDa (beta genes). CaMKII has catalytic and regulatory domains, as well as an ATP-binding domain, and a consensus phosphorylation site. The binding of Ca2+/calmodulin to its regulatory domain releases its auto inhibitory effect and activates the kinase. This kinase activation results in autophosphorylation at threonine 286. The threonine phosphorylation state of CaMKII can be regulated through PP1/PKA. Whereas PP1 (protein phosphatase 1) dephosphorylates phospho-CaMKII at Thr286, PKA (protein kinase A) prevents this dephosphorylation. Autophosphorylation also enables CaMKII to attain an enhanced affinity for NMDA receptors in postsynaptic densities.

克隆：单克隆 22B1

同种型：IgG1

宿主：Mouse

别名：CSAID Binding protein 1 Antibody, CSBP1 Antibody, CSBP2 Antibody, EXIP Antibody, MAP kinase MXI2 Antibody, MAPkinase p38alpha Antibody, MAPK14 Antibody, p38 ALPHA Antibody, p38 MAP kinase Antibody, p38 mitogen activated protein kinase Antibody, RK Antibody, SAPK 2A Antibody, Stress activated protein kinase 2A Antibody

分子量：73 kDa

种属反应性：Human, Mouse, Rat

经测试应用：WB，IHC-P/IHC-Fr，IF/ICC，IP，ELISA

浓度：1mg/ml

纯化方式：Protein G Purified.

抗原：Synthetic peptide to Rat CaMKII.

浓度：WB：1:500-1000    IHC-P/IHC-Fr：1:50-200      IF/ICC：1:500-1000

存储液：PBS pH7.4, 50% glycerol, 0.09% sodium azide.

储存温度：-20℃,避免污染

Western Blot analysis of Mouse Ventricle lysates showing detection of CaMKII protein using Mouse monoclonal CaMK II Antibody at 1:1000. Analysis of CaMKII and NFAT phosphorylation in ventricles of 14 day old mice over-expressing CaMK.

Immunocytochemistry/Immunofluorescence analysis using Mouse monoclonal CaMK II Antibody. Tissue: dissociated hippocampal neurons. Species: Rat. Fixation: Cold 4% paraformaldehyde/0.2% glutaraldehyde in 0.1M sodium phosphate buffer. Primary Antibody: Mouse monoclonal CaMK II Antibody at 1:1000 for 12 hours at 4°C. Secondary Antibody: FITC Goat Anti-Mouse IgG (green) at 1:50 for 30 minutes at RT. Magnification: 10X. 

Immunohistochemistry analysis using Mouse monoclonal CaMK II Antibody. Tissue: backskin. Species: Mouse. Fixation: Bouin’s Fixative and paraffin-embedded. Primary Antibody: Mouse monoclonal CaMK II Antibody at 1:100 for 1 hour at RT. Secondary Antibody: FITC Goat Anti-Mouse (green) at 1:50 for 1 hour at RT. Localization: Muscle, hair follicle, epidermis. Backskin obtained from transgenic mice.

Immunohistochemistry analysis using Mouse monoclonal CaMK II Antibody. Tissue: colon carcinoma. Species: Human. Fixation: Formalin. Primary Antibody: Mouse monoclonal CaMK II Antibody at 1:5000 for 12 hours at 4°C. Secondary Antibody: Biotin Goat Anti-Mouse at 1:2000 for 1 hour at RT. Counterstain: Mayer Hematoxylin (purple/blue) nuclear stain at 200 µl for 2 minutes at RT. Magnification: 40x.