名称：小鼠单克隆HERC5抗体(K29053_10C7)

描述：This gene is a member of the HERC family of ubiquitin ligases and encodes a protein with a HECT domain and five RCC1 repeats. Pro-inflammatory cytokines upregulate expression of this gene in endothelial cells. The protein localizes to the cytoplasm and perinuclear region and functions as an interferon-induced E3 protein ligase that mediates ISGylation of protein targets. The gene lies in a cluster of HERC family genes on chromosome 4.

克隆：单克隆

别称：E3 ISG15--protein ligase HERC5, E3 ISG15 protein ligase HERC5, Cyclin-E-binding protein 1, Cyclin E binding protein 1, CEBP1

纯化方式：Protein A

宿主：Mouse/IgG2a

种属反应性：Human

经测试应用：WB，IHC-P，IP

浓度：1mg/ml

预测分子量： 117 kDa

抗原：Recombinant human HERC5 (immunogen range is 671-1020).

应用：  WB：1:1000     IHC-P：1:200     IP：1:100

存储形式：Liquid

存储液：PBS (pH 7.3) With 0.09% sodium azide

储存温度：-20℃

Western blot analysis of HERC5 in 15 µg of HEK-293 lysate. Sample was run on 6-18% SDS-PAGE under reducing conditions, blotted onto nitrocellulose membrane, and peroxidase conjugated goat anti-mouse IgG was used as the secondary antibody. HERC5 band was visualized using ECL Western Blotting Substrate（PC-90002）. Incubation with primary Mouse Monoclonal HERC5 antibody (K29053_10C7) at a dilution of 1 µg/mL was used.

Immunohistochemistry analysis of HERC5 in paraffin-embedded testis tissue. Sample was incubated with Mouse Monoclonal HERC5 antibody (K29053_10C7) at a dilution of 5 µg/mL (RT, 1 hour). Antigen was retrieved through addition of boiling Tris/EDTA buffer pH 9 in a pressure cooker for 3 min. Endogenous peroxidase activity was quenched by incubating the sections with 3% H2O2 for 30 min at room temperature. Poly-peroxidase conjugated goat anti-mouse IgG was used as the secondary antibody. Diaminobenzidine was used as the chromogen. The section was counterstained with hematoxylin. A tissue section incubated with phosphate-buffered saline followed by incubation with the secondary antibody was used as the background control.

Immunoprecipitation of HERC5 in 200 µg of HEK-293 lysate. Samples are as follows: Lane 1: HEK-293 lysate, Lane 2: HERC5 immunoprecipitated from HEK-293 lysate, Lane3: The same as Lane 2 but KT82 was used as IgG isotype control antibody. After absorption with Protein G beads, the mixture was run on 6-18% SDS-PAGE, blotted onto nitrocellulose membrane, and peroxidase conjugated goat anti-mouse IgG was used as the secondary antibody. The isotype control antibody was KT82. Incubation of samples with Mouse Monoclonal HERC5 antibody (K29053_10C7) at a dilution of 2.5 µg was used.