名称：兔单克隆NF-kB p65抗体

描述：Proteins encoded by the v-Rel viral oncogene and its cellular homolog, c-Rel, are members of a family of transcription factors that include the two subunits of the transcription factor NF kB (p50 and p65) and the Drosophila maternal morphogen, dorsal. Both proteins specifically bind to DNA sequences that are the same or slight variations of the 10 bp kB sequence in the immunoglobulin k light chain enhancer. This same sequence is also present in a number of other cellular and viral enhancers. The DNA binding activity of NFkB is activated and NFkB is subsequently transported from the cytoplasm to the nucleus in cells exposed to mitogens or growth factors. cDNAs encoding precursors for two distinct proteins of the same size have been described, designated p105 and p100. The p105 precursor contains p50 at its N-terminus and a C-terminal region that when expressed as a separate molecule, designated pdI, binds to p50 and regulates its activity.

克隆：单克隆

别称：

NF kappa B p65delta3 antibody

MGC131774 antibody

纯化方式：Protein A affinity purified.

宿主：Rabbit/IgG

经测试应用：WB，IHC-P

浓度：1mg/ml

预测分子量：65kDa

抗原：Synthetic peptide within human RELA aa 490-540.

阳性对照：A549 cell lysate, MCF7 cell lysate, HeLa cell lysate, RAW264.7 cell lysate

应用：  WB：1:500-1000    IHC-P：1:100-500   

存储形式：Liquid

存储液：PBS (pH7.4), 0.1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

储存温度：-20℃

Western blot analysis of NF-kB p65 on different lysates with Rabbit anti-NF-kB p65 antibody at 1/5,000 dilution.

Lysates/proteins at 15 µg/Lane.

Immunohistochemical analysis of paraffin-embedded human lung cancer tissue with Rabbit anti-NF-kB p65 antibody at 1/200 dilution.