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Rabbit Monoclonal CD3 antibody

目录号: PC-74833

The CD3 protein is a T-cell marker, a complex of four structurally distinct membrane glycoprotein isoforms, 20-50 kDa, comprising extracellular, transmembrane and intracellular domains. The CD3 complex is responsible for mediating signal transduction to the internal environment upon antigenic recognition by TCR, causing T-cell proliferation and release of cytokines. 

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规格 价格 库存
50ul 1800.00 现货
100ul 3200.00 现货

基本信息

名称:兔单克隆 CD3 抗体

描述:The CD3 protein is a T-cell marker, a complex of four structurally distinct membrane glycoprotein isoforms, 20-50 kDa, comprising extracellular, transmembrane and intracellular domains. The CD3 complex is responsible for mediating signal transduction to the internal environment upon antigenic recognition by TCR, causing T-cell proliferation and release of cytokines. 

克隆:单克隆

纯化方式:Protein A affinity purified.

宿主:Rabbit/IgG

浓度:1mg/ml

种属反应性:Human

经测试应用:WB,IHC-P/IHC-Fr,IF/ICC,IP

预测分子量:23kDa

阳性对照:Jurkat cell lysates, human lymph nodes tissue, human spleen tissue, human small cell lung cancer, human non-small cell lung cancer, human gastric cancer.

抗原:Synthetic peptide within human CD3E aa 158-207/207.

应用:  WB:1:1000-2000        IHC-P/IHC-Fr:1:100-500       IF/ICC:1:100  

存储形式:Liquid

别名:4930549J05Rik antibody

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亚细胞定位:Cell membrane.

存储液:1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

储存温度:-20℃

 

 

 

Western blot analysis of CD3 on Jurkat cell lysates with Rabbit anti-CD3 antibody at 0.5μg/ml dilution.

 

 

 

 

 

 

 

 

 

Immunohistochemical analysis of paraffin-embedded human lymph nodes tissue with Rabbit anti-CD3 antibody at 1/600 dilution.

 

 

 

 

 

 

 

 

 

 

 

Immunocytochemistry analysis of Jurkat cells labeling CD3 with Rabbit anti-CD3 antibody at 1/100 dilution.

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

Immunofluorescence analysis of paraffin-embedded human lymph nodes tissue labeling CD3 with Rabbit anti-CD3 antibody at 1/100 dilution.

 

 

 

 

 

 

 

 

 

 

CD3 was immunoprecipitated from 1mg/ml Jurkat whole cell lysate with Rabbit Monoclonal CD3 antibody at 2ug/ml dilution. Western blot was performed from the immunoprecipitate using Rabbit Monoclonal CD3 antibody at 1ug/ml dilution. Goat anti-Rabbit IgG-HRP antibody was used as the secondary antibody at 1/300,000 dilution.
Lane 1: Jurkat whole cell lysate 5 μg (Input).
Lane 2: Rabbit Monoclonal CD3 antibody IP in Jurkat whole cell lysate.
Lane 3: Rabbit monoclonal IgG instead of Rabbit Monoclonal CD3 antibody in Jurkat whole cell lysate.
Blocking and dilution buffer and concentration: 5% NFDM/TBST.
Exposure time: 30 seconds.

 

 

 

 

存储温度 -20℃

相关说明文档

储备液配置

注:请根据所选浓度进行配置储备液,配置好储备液后进行分装低温保存,避免反复冻融!PCM提供多种溶剂,如:DMSO(PC-57930);玉米油(PC-86094);棉籽油(PC-30875)等

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