Rabbit Monoclonal MMP-3 antibody
The matrix metalloproteinases (MMP) are a family of peptidase enzymes responsible for the degradation of extracellular matrix components, including collagen, gelatin, fibronectin, laminin and proteoglycan. Transcription of MMP genes is differentially activated by phorbol ester, lipopolysaccharide (LPS) or staphylococcal enterotoxin B (SEB). MMP catalysis requires both calcium and zinc. MMP-3, MMP-10 and MMP-11 (also designated stromelysin-1, -2 and -3 respectively) activate procollagenase. MMP-3 activation of procollagenase can occur via two pathways. Direct activation by MMP-3 is slow and activation by MMP-3 in conjunction with tissue or plasma proteinases is rapid.
| 规格 | 价格 | 库存 |
|---|---|---|
| 50ul | 1800.00 | 现货 |
| 100ul | 3200.00 | 现货 |
基本信息
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名称：兔单克隆 MMP3 抗体 描述：The matrix metalloproteinases (MMP) are a family of peptidase enzymes responsible for the degradation of extracellular matrix components, including collagen, gelatin, fibronectin, laminin and proteoglycan. Transcription of MMP genes is differentially activated by phorbol ester, lipopolysaccharide (LPS) or staphylococcal enterotoxin B (SEB). MMP catalysis requires both calcium and zinc. MMP-3, MMP-10 and MMP-11 (also designated stromelysin-1, -2 and -3 respectively) activate procollagenase. MMP-3 activation of procollagenase can occur via two pathways. Direct activation by MMP-3 is slow and activation by MMP-3 in conjunction with tissue or plasma proteinases is rapid. 克隆：单克隆 纯化方式：Protein A affinity purified. 宿主：Rabbit/IgG 浓度：1mg/ml 种属反应性：Human，Mouse，Rat 经测试应用：WB，IHC-P 预测分子量：54kDa 阳性对照：Human liver tissue lysate, rat liver tissue lysate, rat kidney tissue, human liver tissue, human placenta tissue, mouse liver tissue. 抗原：Synthetic peptide within C-terminal human MMP3. 应用： WB：1:500-2000 IHC-P：1:50-200 存储形式：Liquid 别名：CHDS6 antibody Matrix metalloproteinase 3 antibody Matrix metalloproteinase-3 antibody MGC126102 antibody MGC126103 antibody MGC126104 antibody MMP 3 antibody MMP-3 antibody MMP3 antibody MMP3_HUMAN antibody Proteoglycanase antibody SL-1 antibody SL1 antibody STMY antibody STMY1 antibody STR1 antibody Stromelysin 1 antibody Stromelysin-1 antibody Transin 1 antibody Transin-1 antibody 亚细胞定位：extracellular matrix. 存储液：1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. 储存温度：-20℃
Western blot analysis of MMP-3 on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (PC-80001) at 1:5,000 dilution was used for 1 hour at room temperature.
Western blot analysis of MMP-3 on rat kidney tissue lysates with Rabbit anti-MMP-3 antibody at 1/1,000 dilution.
Immunohistochemical analysis of paraffin-embedded human liver tissue using anti-MMP-3 antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (1/50) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
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| 存储温度 | -20℃ |
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