名称：兔单克隆 Vimentin 抗体

描述：Vimentin (57 kDa) is the most ubiquituos intermediate filament protein and the first to be expressed during cell differentiation. All primitive cell types express vimentin but in most non-mesenchymal cells it is replaced by other intermediate filament proteins during differentiation. Vimentin is expressed in a wide variety of mesenchymal cell types: fibroblasts, endothelial cells etc., and in a number of other cell types derived from mesoderm, e.g., mesothelium and ovarian granulosa cells. Vimentin is present in many different neoplasms but is particulary expressed in those originated from mesenchymal cells.

克隆：单克隆

纯化方式：Protein A affinity purified.

宿主：Rabbit/IgG

浓度：1mg/ml

种属反应性：Human，Mouse，Rat

经测试应用：WB，IHC-P/IHC-Fr，IF/ICC，IP，FC

预测分子量：54kDa

阳性对照：HeLa cell lysate, HEK-293 cell lysate, Jurkat cell lysate, NIH/3T3 cell lysate, C6 cell lysate, C2C12 cell lysate, RAW264.7 cell lysate, C6, HeLa, L6, C2C12, human endometrial carcinoma tissue, mouse colon tissue, mouse cerebellum tissue, human breast carcinoma tissue, human colon tissue, human kidney tissue, mouse large intestine tissue.

抗原：Synthetic peptide within C-terminal human Vimentin.

应用：  WB：1:10000-50000        IHC-P/IHC-Fr：1:1000-2000       IF/ICC：1:100-500      FC：1:1000

存储形式：Liquid

组织特异性：Highly expressed in fibroblasts, some expression in T- and B-lymphocytes, and little or no expression in Burkitt's lymphoma cell lines. Expressed in many hormone-independent mammary carcinoma cell lines.

别名：CTRCT30 antibody

Epididymis luminal protein 113 antibody

FLJ36605 antibody

HEL113 antibody

VIM antibody

VIME_HUMAN antibody

Vimentin antibody

亚细胞定位：Cytoplasm.

存储液：1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

储存温度：-20℃

Western blot analysis of Vimentin on different lysates with Rabbit Monoclonal Vimentin antibody at 1/20,000 dilution.

Immunocytochemistry analysis of HeLa cells labeling Vimentin with Rabbit Monoclonal Vimentin antibody at 1/200 dilution.

Immunohistochemical analysis of paraffin-embedded mouse cerebellum tissue with Rabbit Monoclonal Vimentin antibody at 1/5,000 dilution.

Application: IHC-Fr
Species: Mouse
Site: Cerebellum
Sample: Frozen section
Antibody concentration: 1/2,000
Antigen retrieval: Not required

Vimentin was immunoprecipitated in 0.2mg HeLa cell lysate with Rabbit Monoclonal Vimentin antibody at 2 µg/10 µl beads. Western blot was performed from the immunoprecipitate using Rabbit Monoclonal Vimentin antibody at 1/10,000 dilution. Anti-Rabbit IgG for IP Nano-secondary antibody (PC-80955) at 1/5,000 dilution was used for 1 hour at room temperature.Lane 1: HeLa cell lysate (input)
Lane 2: Rabbit IgG instead of Rabbit Monoclonal Vimentin antibody in HeLa cell lysate
Lane 3: Rabbit Monoclonal Vimentin antibody IP in HeLa cell lysate
Blocking/Dilution buffer: 5% NFDM/TBST
Exposure time: 5 seconds