名称：兔多克隆 NCR1/NKp46 抗体

描述：NKp46（也称为 NCR1 或 CD335）是由 NCR1 基因编码的一种 46 kDa 的糖蛋白，属于免疫球蛋白（Ig）超家族。 它由两个 C2 型的胞外 Ig 样结构域组成，并通过其带正电荷的跨膜结构域与信号适配蛋白相互作用。 NKp46 是人类 NK 细胞的主要激活受体，在哺乳动物中高度保守，广泛表达于 NK 细胞、ILC1、NCR+ ILC3 和少量 T 细胞中。

宿主：Rabbit / IgG

别名：Natural cytotoxicity triggering receptor 1; Lymphocyte antigen 94 homolog; NK cell-activating receptor; Natural killer cell p46-related protein; NK-p46; NKp46; hNKp46; CD antigen CD335

种属反应性：Human，Mouse，Rat

经测试应用：WB，IHC-P

浓度：500ug/ml

抗原：E. coli-derived human NCR1 recombinant protein (Position: Q22-R258).

特异性：This antibody is reactive to NCR1 in Human, Mouse, Rat

分子量：~34

稀释比： WB：1:200-1000     IHC-P：1:50-500

存储液： PBS, 0.02% NaN3, 1 mg/ml BSA and 50% glycerol.

储存温度：-20℃

Western blot analysis of NCR1 using anti-NCR1 antibody 
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions.
Lane 1: rat spleen tissue lysates,
Lane 2: mouse spleen tissue lysates.

IHC analysis of NCR1 using anti-NCR1 antibody
NCR1 was detected in paraffin-embedded section of rat spleen tissue . Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-NCR1 Antibody overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) with DAB as the chromogen.

IHC analysis of NCR1 using anti-NCR1 antibody
NCR1 was detected in paraffin-embedded section of mouse spleen tissue . Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-NCR1 Antibody overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) with DAB as the chromogen.

IHC analysis of NCR1 using anti-NCR1 antibody
NCR1 was detected in paraffin-embedded section of mouse lung tissues. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-NCR1 Antibody overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) with DAB as the chromogen.

IHC analysis of NCR1 using anti-NCR1 antibody
NCR1 was detected in paraffin-embedded section of human tonsil tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-NCR1 Antibody overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) with DAB as the chromogen.