名称：兔多克隆 F-Actin 抗体

描述：Actin is a highly conserved protein and an essential component of cell cytoskeleton and plays an important role in cytoplasmic streaming, cell shape determination, cell division, organelle movement and extension growth. Preferentially expressed in young and expanding tissues, floral organ primordia, developing seeds and emerging inflorescence. Antibodies against plant Actin are useful as loading controls for Western Blotting.

克隆：多克隆

纯化方式：affinity purified by Protein A

宿主：Rabbit/IgG

浓度：1mg/ml

种属反应性：Human，Mouse，Rat

经测试应用：WB，IHC-P/IHC-Fr，IF/ICC

预测分子量：~42kDa

抗原：Synthetic MAP peptide derived from human beta-Actin : 1-50/374

应用：  WB：1:500-2000    IHC-P/IHC-Fr：1:100-500      IF：1:100-500

存储形式：Liquid

组织特异性：Ubiquitously expressed in all eukaryotic cells.

亚细胞定位：Cytoplasm, cytoskeleton.

存储液：0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.

储存温度：-20℃

Sample: Lane1:Hela (Human) Whole Cell Lysate at 30 ug
Lane2:Jurkat (Human) Whole Cell Lysate at 30 ug
Lane3:293T (Human) Whole Cell Lysate at 30 ug
Lane4:Hepg2 (Human) Whole Cell Lysate at 30 ug
Lane5:A431 (Human) Whole Cell Lysate at 30 ug
Lane6:A549 (Human) Whole Cell Lysate at 30 ug
Lane7:H9C2 (Rat) Whole Cell Lysate at 30 ug
Lane8:Testis (Mouse) Tissue Lysate at 30 ug
Primary: Anti-F-Actin at 1/300 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 42 kD
Observed band size: 42 kD

Paraformaldehyde-fixed, paraffin embedded (rat stomach); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (F-Actin) Polyclonal Antibody, Unconjugated at 1:200 overnight at 4°C, followed by operating according to SP Kit(Rabbit) instructionsand DAB staining.

Paraformaldehyde-fixed, paraffin embedded (rat skeletal muscle); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (F-Actin) Polyclonal Antibody, Unconjugated at 1:200 overnight at 4°C, followed by operating according to SP Kit(Rabbit) instructionsand DAB staining.