名称：鸡多克隆mCherry抗体

描述：mCherry是一种红色来自蘑菇珊瑚的红色荧光蛋白，经常用于标记和示踪某些分子和细胞组份。

克隆：多克隆

浓度：200ug/ml

免疫原：Purified recombinant mCherry (Uniprot X5DSL3) expressed in E.Coli

特异性：此抗体对tdTomato和mCherry蛋白具有特异性，不会与GFP发生交叉反应

同种型：IGY

宿主：Chicken

种属反应性：All

经测试应用：WB，IF/ICC，IHC-P，IHC-Fr

预测分子量：27 KDa for native monomer

浓度：WB：1:500-1000   IF/ICC：1:100-500    IHC-P/IHC-Fr：1:50-400 

存储液：PBS with 20% glycerol and 0.02% sodium azide

储存温度：-20℃（1周以上）,避免污染

Western blot of COS7 cells transfected with mCherry-myc-FLAG (lane 1), GFP (lane 2), or mock transfected cells (lane 3) using chicken α-mCherry antibody showing the expected staining for mCherry in lane 1 and no staining in GFP or untransfected cells. The ~37kDa major band in lane 1 represents full-length mCherry-myc-FLAG while the shorter ~24kDa band represents an mCherry degradation product.

Immunostaining of postnatal day 12 Scube2-CreER ROSA26-tdTomato mouse lung tissues fixed in 4% paraformaldehyde and paraffin embedded (FFPE) showing specific detection of tdTomato in the mesenchyme using mCherry ( 1:100; green). Nkx2.1 (red) marks the epithelium and nuclei are labeled with DAPI (blue). 

IF of a section of mouse brain NAc labeled with Anti-mCherry (1:1000, red), colabeled with eGFP, green, and DAPI staining of nuclear DNA. The Anti-mCherry specifically targeted the cpmApple-based membrane-localized red dopamine sensor co-expressed with eGFP in NAc and identified both cellular and subcellular distribution of the sensor. 20X/0.8 Air & 40X/1.1 Water Confocal, scale bar 100 um; 20 um. Donkey anti-chicken AlexaFluor647.