名称：鸡多克隆Lamin A/C抗体

描述：Lamin A and lamin C are members of the intermediate filament protein family and are located in the nucleus where they function as skeletal components of the inner nuclear membrane . The two proteins are generated by alternate transcription from the single LMNA gene. Lamin A has a molecular weight of about 74kDa while lamin C is 65kDa. The lamin A protein includes a C-terminal segment of 98 amino acids missing from lamin C, while lamin C has a unique C-terminal 6 amino acid peptide not present in lamin A. As a result antibodies raised against lamin A are almost certain bind to lamin C. During cell division the nuclear lamina breaks down and lamin A/C containing filaments depolymerize, this being regulated by phosphorylation by cyclin dependent protein kinase .

克隆：多克隆

纯化方式：Supplied as an aliquot of concentrated IgY preparation plus 5mM NaN₃

同种型：IGY

宿主：Chicken

种属反应性：Human，Mouse，Rat，Horse，Monkey，Dog

经测试应用：WB，IF/ICC，IHC-P，IHC-Fr

预测分子量：65kDa and 74kDa

抗原：Full length recombinant human lamin A protein expressed in and purified from E. coli.

推荐浓度：WB：1:1000-2000  IF/ICC：1:500-1000   IHC-P/IHC-Fr：1:500-1000

存储液：PBS PH7.2

储存温度：2-8℃,避免污染

使用Chicken Polyclonal Lamin A/C抗体进行WB测试，1:1000稀释（绿色），红色为37kDa内参蛋白GAPDH，泳道1：标准蛋白marker；泳道2：HeLa cytosol ；泳道3：HeLa nuclear ；泳道4：NIH-3T3 cytosol ；泳道5：NIH-3T3 nuclear fractions.65kDa和74kDa处条带分别对应A和C

使用Chicken Polyclonal Lamin A/C抗体进行免疫荧光分析，1:2000稀释（红色），绿色为Actin 1:500稀释，蓝色为细胞核染色。

使用Chicken Polyclonal Lamin A/C抗体进行人大脑皮层切片染色，1:2000稀释，苏木精复染。