名称：兔多克隆LPSAT抗体

描述：Enables 1-acylglycerophosphocholine O-acyltransferase activity; 1-acylglycerophosphoethanolamine O-acyltransferase activity; and 1-acylglycerophosphoserine O-acyltransferase activity. Involved in phosphatidylcholine acyl-chain remodeling; phosphatidylethanolamine acyl-chain remodeling; and phosphatidylserine acyl-chain remodeling. Located in endoplasmic reticulum membrane.

克隆：多克隆

纯化方式：Affinity purification

宿主：Rabbit/IgG

别名：

种属反应性：Human，Mouse，Rat

经测试应用：WB，IHC-P，ELISA，IP(mouse)

分子量：56kDa

抗原：Recombinant fusion protein containing a sequence corresponding to amino acids 200-300 of human LPCAT3 (NP_005759.4).

应用：  WB：1:500-1000     IHC-P：1:50-200

存储形式：Liquid

存储液： PBS with 0.09% Sodium azide,50% glycerol,pH7.3.

储存温度：-20℃

Western blot analysis of lysates from HepG2 cells, using Rabbit Polyclonal LPSAT Antibody at 1:1000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (PC-80001) at 1:10000 dilution. Lysates/proteins: 25μg per lane.

Blocking buffer: 3% nonfat dry milk in TBST.

Detection: Western Quick HRP substrate (PC-90002).

Exposure time: 30s

Immunohistochemistry analysis of paraffin-embedded Rat kidney tissue using Rabbit Polyclonal LPSAT Antibody at a dilution of 1:100 (40x lens). High pressure antigen retrieval was performed with 0.01 M citrate buffer (pH 6.0) prior to IHC staining.

Immunohistochemistry analysis of paraffin-embedded Mouse kidney tissue using Rabbit Polyclonal LPSAT Antibody at a dilution of 1:100 (40x lens). High pressure antigen retrieval was performed with 0.01 M citrate buffer (pH 6.0) prior to IHC staining.

Immunohistochemistry analysis of paraffin-embedded Human liver tissue usingRabbit Polyclonal LPSAT Antibody at a dilution of 1:100 (40x lens). High pressure antigen retrieval was performed with 0.01 M citrate buffer (pH 6.0) prior to IHC staining.