Rabbit Polyclonal VDAC1 / Porin Antibody
Voltage-dependent anion-selective channel (VDAC1) (also referred to as porin, isoform 1) is a small protein, originally discovered in the outer membrane of mitochondria where it constitutes the major pore-forming protein. The porin protein VDAC1 allows to the outer-most membrane of the mitochondrion free permeability to low molecular-weight solutes. VDAC1 has been shown to co-immunoprecipitate with the anti-apoptotic protein Bcl-2 and suggested to be involved in forming the mitochondrial pore which releases cytochrome c during apoptosis.
| 规格 | 价格 | 库存 |
|---|---|---|
| 50ul | 1300.00 | 现货 |
| 100ul | 2200.00 | 现货 |
基本信息
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名称：兔多克隆SVDAC1抗体 描述：Voltage-dependent anion-selective channel (VDAC1) (also referred to as porin, isoform 1) is a small protein, originally discovered in the outer membrane of mitochondria where it constitutes the major pore-forming protein. The porin protein VDAC1 allows to the outer-most membrane of the mitochondrion free permeability to low molecular-weight solutes. VDAC1 has been shown to co-immunoprecipitate with the anti-apoptotic protein Bcl-2 and suggested to be involved in forming the mitochondrial pore which releases cytochrome c during apoptosis. 克隆：多克隆 纯化方式：Affinity purification 宿主：Rabbit/IgG 浓度：1mg/ml 别名：hVDAC1 antibody 种属反应性：Human，Mouse，Zebrafish 经测试应用：WB，IHC-P 分子量：731kDa 抗原：Recombinant fusion protein containing a sequence corresponding to amino acids 1-283 of human VDAC1 / Porin (NP_003365.1). 应用： WB：1:500-1000 IHC-P：1:50-200 存储形式：Liquid 存储液：PBS with 0.02% sodium azide,50% glycerol,pH7.3. 储存温度：-20℃
Western blot analysis of various lysates using Rabbit Polyclonal VDAC1 / Porin Antibody at 1:400 dilution.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (PC-80001) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: Western Quick HRP substrate (PC-90002).
Exposure time: 1s.
 Western blot analysis of various lysates using Rabbit Polyclonal VDAC1 / Porin Antibody at 1:400 dilution.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (PC-80001) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: Western Quick HRP substrate (PC-90002).
Exposure time: 1s.
Immunohistochemistry analysis of paraffin-embedded Rat liver using Rabbit Polyclonal VDAC1 / Porin Antibody at dilution of 1:20 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.
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| 存储温度 | -20℃ |
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