Rabbit Polyclonal SDHA Antibody
This gene encodes a major catalytic subunit of succinate-ubiquinone oxidoreductase, a complex of the mitochondrial respiratory chain. The complex is composed of four nuclear-encoded subunits and is localized in the mitochondrial inner membrane. Mutations in this gene have been associated with a form of mitochondrial respiratory chain deficiency known as Leigh Syndrome. A pseudogene has been identified on chromosome 3q29.
| 规格 | 价格 | 库存 |
|---|---|---|
| 50ul | 1800.00 | 现货 |
| 100ul | 3200.00 | 现货 |
基本信息
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名称：兔多克隆SDHA抗体 描述：This gene encodes a major catalytic subunit of succinate-ubiquinone oxidoreductase, a complex of the mitochondrial respiratory chain. The complex is composed of four nuclear-encoded subunits and is localized in the mitochondrial inner membrane. Mutations in this gene have been associated with a form of mitochondrial respiratory chain deficiency known as Leigh Syndrome. A pseudogene has been identified on chromosome 3q29. 克隆：多克隆 纯化方式：Affinity purification 宿主：Rabbit/IgG 浓度：1mg/ml 别名：FP; PGL5; SDH1; SDH2; SDHF; CMD1GG 种属反应性：Human，Mouse 经测试应用：WB，IHC，IF/ICC 分子量：73kDa 抗原：Recombinant fusion protein containing a sequence corresponding to amino acids 385-664 of human SDHA (NP_004159.2). 应用： WB：1:500-1000 IHC：1:50-200 IF/ICC：1:50-100 存储形式：Liquid 存储液：PBS with 0.02% sodium azide,50% glycerol,pH7.3. 储存温度：-20℃
Western blot analysis of various lysates using Rabbit Polyclonal SDHA Antibody at 1:1000 dilution.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (PC-80001) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: Western Quick HRP substrate (PC-90002).
Exposure time: 1s.
 Immunohistochemistry analysis of paraffin-embedded Human colon carcinoma using Rabbit Polyclonal SDHA Antibody at dilution of 1:100 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.
 Immunofluorescence analysis of NIH/3T3 cells using Rabbit Polyclonal SDHA Antibody at dilution of 1:100 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.
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| 存储温度 | -20℃ |
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