名称：兔单克隆 alpha Tubulin 抗体

描述：The cytoskeleton consists of three types of cytosolic fibers: microtubules, microfilaments (actin filaments), and intermediate filaments. Globular tubulin subunits comprise the microtubule building block, with α/β-tubulin heterodimers forming the tubulin subunit common to all eukaryotic cells. Acetylation of alpha chains at Lys-40 stabilizes microtubules and affects affinity and processivity of microtubule motors. 

克隆：单克隆

纯化方式：Protein A affinity purified.

宿主：Rabbit/IgG

浓度：1mg/ml

种属反应性：Human，Mouse，Rat

经测试应用：WB，IHC-P，IF/ICC，IP

预测分子量：55kDa

阳性对照：HeLa cell lysate, PC-12 cell lysate, NIH/3T3 cell lysate, Daudi cell lysate, Jurkat cell lysate, A431 cell lysate, K-562 cell lysate, Daudi, K-562, HeLa, NIH/3T3, human tonsil tissue, rat brain tissue.

抗原：Synthetic peptide within Human alpha Tubulin aa 402-451 / 451.

应用：  WB：1:500-2000        IHC-P：1:100-1000       IF/ICC：1:200-500         IP：1-2μg/sample

存储形式：Liquid

别名：Alpha-tubulin antibody

Alpha-tubulin 1 antibody

ALS22 antibody

B ALPHA 1 antibody

bA408E5.3 antibody

H2 ALPHA antibody

Hum a tub1 antibody

Hum a tub2 antibody

LIS3 antibody

存储液：PBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

储存温度：-20℃

Western blot analysis of alpha Tubulin on different lysates with Rabbit anti-alpha Tubulin antibody at 1/5,000 dilution.

Exposure time: 1 minutes 2 seconds; ECL: PC-30002

Immunocytochemistry analysis of NIH/3T3 cells labeling alpha Tubulin with Rabbit anti-alpha Tubulin antibody at 1/500 dilution.

Immunohistochemical analysis of paraffin-embedded human tonsil tissue with Rabbit anti-alpha Tubulin antibody at 1/1,000 dilution.

alpha Tubulin was immunoprecipitated from 0.2 mg HeLa cell lysate with Rabbit Monoclonal alpha Tubulin Antibody at 2 µg/10 µl beads. Western blot was performed from the immunoprecipitate using Rabbit Monoclonal alpha Tubulin Antibody at 1/1,000 dilution. Anti-Rabbit IgG for IP Nano-secondary antibody at 1/5,000 dilution was used for 1 hour at room temperature.

Lane 1: HeLa cell lysate (input)
Lane 2: Rabbit Monoclonal alpha Tubulin Antibody IP in HeLa cell lysate

Lane 3: Rabbit IgG instead of Rabbit Monoclonal alpha Tubulin Antibody in HeLa cell lysateBlocking/Dilution buffer: 5% NFDM/TBST
Exposure time: 2 seconds; ECL: K1801