名称：兔单克隆 COL1A1 抗体

描述：Collagen, type I, alpha 1, also known as alpha-1 type I collagen, is a protein that in humans is encoded by the COL1A1 gene. COL1A1 encodes the major component of type I collagen, the fibrillar collagen found in most connective tissues, including cartilage. Collagen is a protein that strengthens and supports many tissues in the body, including cartilage, bone, tendon, skin and the white part of the eye (sclera). The COL1A1 gene produces a component of type I collagen, called the pro-alpha1(I) chain. This chain combines with another pro-alpha1(I) chain and also with a pro-alpha2(I) chain (produced by the COL1A2 gene) to make a molecule of type I procollagen. These triple-stranded, rope-like procollagen molecules must be processed by enzymes outside the cell. Once these molecules are processed, they arrange themselves into long, thin fibrils that cross-link to one another in the spaces around cells. The cross-links result in the formation of very strong mature type I collagen fibers. Collagenous function includes rigidity and elasticity.

克隆：单克隆

纯化方式：Protein A affinity purified.

宿主：Rabbit/IgG

种属反应性：Human，Mouse，Rat

经测试应用：WB，IHC-P/IHC-Fr，IF-cell，Fc

预测分子量：44kDa

抗原：Synthetic peptide within Human COL1A1 aa 1197-1208.

应用：  WB：1:1000-2000        IHC-P/IHC-Fr：1:1000-2000       IF-cell：1:100-500        Fc：1:500-1000 

存储形式：Liquid

别名：Alpha 1 type I collagen antibody

          alpha1(I) procollagen antibody

          CO1A1_HUMAN antibody

          COL1A1 antibody

          Collagen alpha 1 chain type I antibody

          Collagen alpha-1(I) chain antibody

          Collagen alpha-1(I) chain preproprotein antibody

          Collagen I alpha 1 polypeptide antibody

          Collagen of skin, tendon and bone, alpha-1 chain antibody

          Collagen type I alpha 1 antibody

          EDSC antibody

          OI1 antibody

          OI2 antibody

          OI3 antibody

          OI4 antibody

          pro-alpha-1 collagen type 1 antibody

          type I proalpha 1 antibody

          type I procollagen alpha 1 chain antibody

          Type I procollagen antibody

存储液：PBS (pH7.4), 0.1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

    Western blot analysis of COL1A1 on different lysates with Rabbit Monoclonal COL1A1 Antibody at 1/2,000 dilution.

Left: HFF-1 cell lysate (RIPA buffer)

Right: HFF-1 cell lysate (Hot lysis buffer)

Predicted band size: 139 kDa

Observed band size: 200 kDa

Exposure time: 6 seconds; 

4-20% SDS-PAGE gel.

    Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody at 1/2,000 dilution was used in PC-30016 at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.

    Immunocytochemistry analysis of HFF-1 cells labeling COL1A1 with Rabbit Monoclonal COL1A1      Antibody at 1/500 dilution.

    Cells were fixed in 4% paraformaldehyde for 20 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 5 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit Monoclonal COL1A1 Antibody at 1/500 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI.

                                                               Beta tubulin (red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L                                                                     (iFluor™ 594) was used as the secondary antibody at 1/1,000 dilution.

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue with Rabbit Monoclonal COL1A1 Antibody at 1/1,000 dilution.

    The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

    Immunohistochemical analysis of paraffin-embedded rat kidney tissue with Rabbit Monoclonal COL1A1 Antibody at 1/1,000 dilution.

    The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

Flow cytometric analysis of HFF-1 cells labeling COL1A1.

    Cells were washed twice with cold PBS and resuspend. Then stained with the primary antibody (1μg/mL) (red) compared with Rabbit IgG Isotype Control (green). After incubation of the primary antibody at +4℃ for an hour, the cells were stained with a iFluor™ 488 conjugate-Goat anti-Rabbit IgG Secondary antibody at 1/1,000 dilution for 30 minutes at +4℃. Unlabelled sample was used as a control ( black).