24小时咨询热线
13248283690

Rabbit Monoclonal ATP5O antibody

目录号: PC-68211

ATP synthase subunit O, mitochondrial is an enzyme that in humans is encoded by the ATP5PO gene. The protein encoded by this gene is a component of the F-type ATPase found in the mitochondrial matrix. F-type ATPases are composed of a catalytic core and a membrane proton channel. The encoded protein appears to be part of the connector linking these two components and may be involved in transmission of conformational changes or proton conductance.

PlantChemMed 的所有产品仅供科研使用,不能用于任何个人和临床研究!
规格 价格 库存
50ul 1800.00 现货
100ul 3200.00 现货

基本信息

名称:兔单克隆 ATP50 抗体

描述:ATP synthase subunit O, mitochondrial is an enzyme that in humans is encoded by the ATP5PO gene. The protein encoded by this gene is a component of the F-type ATPase found in the mitochondrial matrix. F-type ATPases are composed of a catalytic core and a membrane proton channel. The encoded protein appears to be part of the connector linking these two components and may be involved in transmission of conformational changes or proton conductance.

克隆:单克隆

纯化方式:Protein A affinity purified.

宿主:Rabbit/IgG

浓度:1mg/ml

种属反应性:Human,Mouse,Rat

经测试应用:WB,IHC-P/IHC-Fr,IF/ICC,IP

预测分子量:23kDa

阳性对照:K-562 cell lysate, HeLa cell lysate, HepG2 cell lysate, A549 cell lysate, Neuro-2a cell lysate, A20 cell lysate, PC-12 cell lysate, HeLa, Neuro-2a, PC-12.

抗原:Recombinant protein within human ATP5O aa 1-213.

应用:  WB:1:500-2000        IHC-P/IHC-Fr:1:100-500      ICC/IF:1:50-100      IP:1-2μg/sample

存储形式:Liquid

别名:ATP synthase O subunit mitochondrial precursor antibody

ATP synthase subunit O antibody

ATP synthase, H+ transporting, mitochondrial F1 complex, O subunit antibody

ATP5O antibody

ATPO antibody

ATPO_HUMAN antibody

mitochondrial antibody

Mitochondrial ATP synthase, O subunit antibody

Oligomycin sensitivity conferral protein antibody

OSCP antibody

存储液:1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

储存温度:-20℃

 

Western blot analysis of ATP5O on different lysates with Rabbit Monoclonal ATP5O antibody at 1/5,000 dilution.

Lysates/proteins at 20 µg/Lane.

 

Exposure time: 6 seconds; ECL: PC-30002;

 

 

 

 

 

 

Immunocytochemistry analysis of HeLa cells labeling ATP5O with Rabbit Monoclonal ATP5O antibody at 1/100 dilution.

 

 

 

 

 

 

 

Immunohistochemical analysis of paraffin-embedded human kidney tissue with Rabbit Monoclonal ATP5O antibody at 1/2,000 dilution.

 

 

 

 

 

 

 

 

 

 

ATP5O was immunoprecipitated from 0.2 mg HeLa cell lysate withRabbit Monoclonal ATP5O antibody at 2 µg/10 µl beads. Western blot was performed from the immunoprecipitate using Rabbit Monoclonal ATP5O antibody  at 1/5,000 dilution. HRP Conjugated Anti-Rabbit IgG for IP Nano-secondary antibody at 1/5,000 dilution was used for 1 hour at room temperature.


Lane 1: HeLa cell lysate (input)
Lane 2: Rabbit Monoclonal ATP5O antibody IP in HeLa cell lysate
Lane 3: Rabbit IgG instead of Rabbit Monoclonal ATP5O antibody  in HeLa cell lysate

Exposure time: 3 minutes; ECL:PC-30002

存储温度 -20℃

相关说明文档

储备液配置

注:请根据所选浓度进行配置储备液,配置好储备液后进行分装低温保存,避免反复冻融!PCM提供多种溶剂,如:DMSO(PC-57930);玉米油(PC-86094);棉籽油(PC-30875)等

相关产品推荐

浏览历史产品