名称：兔单克隆 ABH1 抗体

描述：This gene encodes a homolog to the E. coli alkB gene product. The E. coli alkB protein is part of the adaptive response mechanism of DNA alkylation damage repair. It is involved in damage reversal by oxidative demethylation of 1-methyladenine and 3-methylcytosine. Dioxygenase that repairs alkylated single-stranded DNA and RNA containing 3-methylcytosine by oxidative demethylation. Requires molecular oxygen, alpha-ketoglutarate and iron. May have a role in placental trophoblast lineage differentiation. Has DNA lyase activity and introduces double-stranded breaks at abasic sites. Cleaves both single-stranded DNA and double-stranded DNA at abasic sites, with the greatest activity towards double-stranded DNA with two abasic sites.

克隆：单克隆

纯化方式：Protein A affinity purified.

宿主：Rabbit/IgG

种属反应性：Human，Mouse，Rat

经测试应用：WB，IHC-P/IHC-Fr，IF/ICC

预测分子量：44kDa

抗原：A synthesized peptide derived from human ALKBH1 : 1-85

应用：  WB：1:500-2000        IHC-P/IHC-Fr：1:100-200       IF/ICC：1:100-200    

存储形式：Liquid

别名：ALKB1_HUMAN; ALKBH1; Alkylated DNA repair protein alkB homolog 1; Alpha-ketoglutarate-dependent dioxygenase ABH1; DNA 6mA demethylase; DNA N6-methyl adenine demethylase ALKBH1; DNA lyase ABH1; DNA oxidative demethylase ALKBH1; mRNA N(3)-methylcytidine demethylase; 1.14.11.-; ABH; ABH1; ALKBH; alkB; hABH; alkB homolog 1, histone H2A dioxygenase; alkB, alkylation repair homolog (E. coli); alkB, alkylation repair homolog 1 (E. coli)

存储液：PBS (pH7.4), 1% BSA, 50% Glycerol. Preservative: 0.02% PC300.

储存温度：-20℃

25 ug total protein per lane of various lysates (see on figure) probed with ALKBH1 monoclonal antibody, at 1:1000 dilution and 4°C overnight incubation. Followed by conjugated secondary antibody incubation at room temperature for 60 min.

Paraformaldehyde-fixed, paraffin embedded Rat Colon; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with ALKBH1 Monoclonal Antibody, at 1:200 overnight at 4°C, followed by DAB .

Paraformaldehyde-fixed, paraffin embedded Rat Kidney; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with ALKBH1 Monoclonal Antibody,at 1:200 overnight at 4°C, followed by DAB.

Paraformaldehyde-fixed, paraffin embedded Rat Liver; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with ALKBH1 Monoclonal Antibody,at 1:200 overnight at 4°C, followed by DAB.

Paraformaldehyde-fixed, paraffin embedded Human Liver; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with ALKBH1 Monoclonal Antibody, at 1:200 overnight at 4°C, followed by DAB.

Paraformaldehyde-fixed, paraffin embedded Mouse Colon; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with ALKBH1 Monoclonal Antibody, at 1:200 overnight at 4°C, followed by DAB.

Paraformaldehyde-fixed, paraffin embedded Mouse Kidney; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with ALKBH1 Monoclonal Antibody, at 1:200 overnight at 4°C, followed by DAB.

Paraformaldehyde-fixed, paraffin embedded Human Kidney; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with ALKBH1 Monoclonal Antibody, at 1:200 overnight at 4°C, followed by DAB.