名称：兔多克隆IL-6抗体

描述：Cytokines play an essential role in the final differentiation of B-cells into Ig-secreting cells Involved in lymphocyte and monocyte differentiation. Interleukin-6 (IL-6) is a multifunctional cytokine with a wide variety of biological functions. IL-6 is implicated in the final differentiation of B-cells into immunoglobulin-secreting cells, myeloma and plasmacytoma growth, nerve cell differentiation, and activation of hepatocytes and mitogen-stimulated helper T cells.

克隆：多克隆

纯化方式：Protein A affinity purified.

同种型：IgG

宿主：Rabbit

种属反应性：Human，Mouse

经测试应用：WB，IHC-P

预测分子量：24KDa

免疫原：Recombinant protein within mouse IL6 aa 1-211.

浓度：WB：1:100-1000     IHC-P：1:50-200

存储液：Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.

Western blot analysis of IL-6 on different lysates with Rabbit anti-IL-6 antibody at 1/1,000 dilution.

Immunohistochemical analysis of paraffin-embedded human spleen tissue using anti-IL-6 antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (1/100) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.