名称：兔多克隆RUNX2抗体

描述：Transcription factor involved in osteoblastic differentiation and skeletal morphogenesis. Essential for the maturation of osteoblasts and both intramembranous and endochondral ossification. CBF binds to the core site, 5'-PYGPYGGT-3', of a number of enhancers and promoters, including murine leukemia virus, polyomavirus enhancer, T-cell receptor enhancers, osteocalcin, osteopontin, bone sialoprotein, alpha 1(I) collagen, LCK, IL-3 and GM-CSF promoters.

克隆：多克隆

浓度： 1mg/ml

同种型：IgG

宿主：Rabbit

种属反应性：Human，Mouse，Rat

经测试应用：WB，IHC，IF/ICC

分子量：56kDa,48kDa; 57kD(Calculated)

免疫原：A synthesized peptide derived from human RUNX2, corresponding to a region within the internal amino acids.

特异性：RUNX2 Antibody detects endogenous levels of total RUNX2.

纯化方式：Protein A affinity purified.

别称：OSF 2; OSF-2; OSF2; Osteoblast specific transcription factor 2; Osteoblast-specific transcription factor 2; OTTHUMP00000016533; PEA2 alpha A; PEA2-alpha A; PEA2aA; PEBP2 alpha A

形式：Liquid

浓度：WB：1:500-1000       IHC：1:50-200       IF/ICC：1:100-500

存储液：PBS containing 0.02% sodium azide.

储存温度：-20℃

WB analysis of HeLa cell lysate using RUNX2 antibody. The lane on the left was treated with the antigen-specific peptide.

WB analysis of various tissue lysates using RUNX2 antibody.
Lane 1 : Rat heart tissue lysate
Lane 2 : Rat brain tissue lysate
Lane 3 : Mouse lung tissue lysate
Lane 4 : Mouse brain tissue lysate

WB analysis of various tissue lysates using RUNX2 antibody.
Lane 1 : Mouse brain tissue lysate treated with blocking peptide
Lane 2 : Mouse brain tissue lysate
Lane 3 : Rat spleen tissue lysate

ICC/IF analysis of PFA-fixed HeLa cells using RUNX2 antibody.
Red : Primary antibody
Green : Tubulin
Permeabilization : 0.1% Triton X-100
Dilution : 1:200

IHC-P analysis of rat brain tissue using RUNX2 antibody.
Antigen retrieval : Heat mediated antigen retrieval step in citrate buffer
Dilution : 1:100

IHC-P analysis of human bone marrow tissue using RUNX2 antibody.
Antigen retrieval : Heat mediated antigen retrieval step in citrate buffer
Dilution : 1:100