名称：兔多克隆Ki67抗体

描述：Ki67 antigen is the prototypic cell cycle related nuclear protein, expressed by proliferating cells in all phases of the active cell cycle (G1, S, G2 and M phase). It is absent in resting (G0) cells. Ki67 antibodies are useful in establishing the cell growing fraction in neoplasms (immunohistochemically quantified by determining the number of Ki67 positive cells among the total number of resting cells = Ki67 index). In neoplastic tissues the prognostic value is comparable to the tritiated thymidine labelling index. The correlation between low Ki67 index and histologically low grade tumours is strong. Ki67 is routinely used as a neuronal marker of cell cycling and proliferation. 

克隆：多克隆

纯化方式：The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95%.

同种型：IgG1

浓度：1mg/ml

宿主：Rabbit

种属反应性：Human,Mouse

经测试应用：WB，IF/ICC，IHC

预测分子量：359KDa

抗原：Recombinant fusion protein of human Ki67.

浓度：WB：1:500-1000    IF/ICC：1:50-100     IHC-P：1:50-100

存储液：PBS (pH 7.3) containing 1% BSA, 50% glycerol and 0.02% sodium azide.

Western blot analysis of Ki67 on HepG2 cell lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (PC-65132, 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:5,000 dilution was used for 1 hour at room temperature. 

  Immunofluorescence analysis of U-2 OS cells using Ki67 Rabbit pAb at dilution of 1:100 . Blue: DAPI    for nuclear staining.