产品描述：Interleukin 13 (IL-13) with a molecular mass of 10 kDa cytokine, it secreted by T helper type 2 (Th2) cells, CD4 cells, natural killer T cell, mast cells, basophils, eosinophils and nuocytes. It is homologous to IL-4 and shares many of its biologic activities on mononuclear phagocytic cells, endothelial cells, epithelial cells, and B cells.

蛋白编号：P35225

别名：interleukin 13, NC30, IL13

分子量：The protein has a calculated MW of 13.28 kDa. The protein migrates as 15 kDa under reducing condition (SDS-PAGE analysis).

内毒素：<0.01 EU per 1 μg of the protein by the LAL method.

蛋白序列：MGPVPPSTALRELIEELVNITQNQKAPLCNGSMVWSINLTAGMYCAALESLINVSGCSAIEKTQRMLSGFCPHKVSAGQFSSLHVRDTKIEVAQFVKDLLLHLKKLFREGRFN with polyhistidine tag at the C-terminus

蛋白标签：His Tag (C-term)

活性：Measure by its ability to induce TF-1 cells proliferation. The ED₅₀ for this effect is<0.8 ng/mL. The specific activity of recombinant human IL-13 is approximately >1 x10⁶ IU/ mg.

纯度：>95% as determined by SDS-PAGE.

表达系统：Escherichia coli

溶剂：The protein was lyophilized from a 0.2 µm filtered solution containing 1X PBS, pH 7.4. 

干粉复溶：

Always centrifuge tubes before opening.Do not mix by vortex or pipetting.

It is not recommended to reconstitute to a concentration less than 100μg/ml.

Dissolve the lyophilized protein in distilled water.

Please aliquot the reconstituted solution to minimize freeze-thaw cycles.

保存条件：Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt.

Reconstituted protein solution can be stored at 2-8°C for 2-7 days.

Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months.