产品描述：Vascular Endothelial Growth Factors 165 (VEGF165) is a potent growth and angiogenic cytokine which belongs to the VEGF family, includes VEGF-A, VEGF-B, VEGF-C, VEGF-D, VEGF-E, and PIGF. VEGF165 is an abundant glycosylated cytokine composed of two identical 165 amino acid chains. VEGF165 plays an important role in embryonic vasculogenesis, angiogenesis and neurogenesis.

蛋白编号：Q00731(Mouse), P16612(Rat)

别名：vascular endothelial growth factor 165, VPF, Folliculostellate cell-derived growth factor, Glioma-derived endothelial cell mitogen

分子量：The protein has a calculated MW of 20.22 kDa. The protein migrates as 18 kDa under reducing condition (SDS-PAGE analysis).

内毒素：<0.01 EU per 1 μg of the protein by the LAL method.

蛋白序列：MAPTTEGEQKSHEVIKFMDVYQRSYCRPIETLVDIFQEYPDEIEYIFKPSCVPLMRCAGCCNDEALECVPTSESNITMQIMRIKPHQSQHIGEMSFLQHSRCECRPKKDRTKPENHCEPCSERRKHLFVQDPQTCKCSCKNTDSRCKARQLELNERTCRCDKPRR with polyhistidine tag at the C-terminus.

蛋白标签：His Tag (C-term)

活性：Measure by its ability to induce proliferation in HUVEC cells. The ED₅₀ for this effect is <10 ng/mL.

纯度：>98% as determined by SDS-PAGE.

表达系统：Escherichia coli

溶剂：The protein was lyophilized from a 0.2 µm filtered solution containing 50 mM Tris, 500 mM NaCl, pH 8.5.

干粉复溶：

Always centrifuge tubes before opening.Do not mix by vortex or pipetting.

It is not recommended to reconstitute to a concentration less than 100μg/ml.

Dissolve the lyophilized protein in distilled water.

Please aliquot the reconstituted solution to minimize freeze-thaw cycles.

保存条件：Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt.

Reconstituted protein solution can be stored at 2-8°C for 2-7 days.

Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months.