产品描述：Interleukin-10 (IL-10), also known as cytokine synthesis inhibitory factor (CSIF), is an anti-inflammatory cytokine. Many different types of cells can produce IL-10, including immune cells and non-immune cells. IL-10 exerts inhibitory functions on DCs and macrophages, which is a potent inhibitor of antigen presentation and limit the production of the Th1-associated cytokines IL-2 and interferon-γ (IFN-γ). IL-10 is also a key immunoregulator during infection due to its inhibitory effect on inflammatory cytokine production. Consequently, the excessive Th1 and CD8+ T cell responses could be suppressed during infection.

蛋白编号：P18893

别名：interleukin 10, B-TCGF, CSIF, TGIF, IL10

分子量：The protein has a calculated MW of 19.7 kDa. The protein migrates as 19 kDa under reducing condition (SDS-PAGE analysis).

内毒素：<0.01 EU per 1 μg of the protein by the LAL method.

蛋白序列：MSRGQYSREDNNCTHFPVGQSHMLLELRTAFSQVKTFFQTKDQLDNILLTDSLMQDFKGYLGCQALSEMIQFYLVEVMPQAEKHGPEIKEHLNSLGEKLKTLRMRLRRCHRFLPCENKSKAVEQVKSDFNKLQDQGVYKAMNEFDIFINCIEAYMMIKMKS with polyhistidine tag at the C-terminus.

蛋白标签：His Tag (C-term)

活性：Measure by its ability to induce MC/9‑2 cells proliferation. The ED₅₀ for this effect is <1 ng/mL. The specific activity of recombinant mouse IL-10 is > 1 x 10⁶ IU/mg.

纯度：>98% as determined by SDS-PAGE.

表达系统：Escherichia coli

溶剂：The protein was lyophilized from a 0.2 µm filtered solution containing 1X PBS, pH 7.4. 

干粉复溶：

Always centrifuge tubes before opening.Do not mix by vortex or pipetting.

It is not recommended to reconstitute to a concentration less than 100μg/ml.

Dissolve the lyophilized protein in distilled water.

Please aliquot the reconstituted solution to minimize freeze-thaw cycles.

保存条件：Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt.

Reconstituted protein solution can be stored at 2-8°C for 2-7 days.

Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months.