名称：兔多克隆 IL-1b 抗体

描述：Thiol protease that cleaves IL-1 beta between an Asp and an Ala, releasing the mature cytokine which is involved in a variety of inflammatory processes. Important for defense against pathogens. Cleaves and activates sterol regulatory element binding proteins (SREBPs). Can also promote apoptosis.

克隆：多克隆

浓度： 1mg/ml

同种型：IgG

宿主：Rabbit

种属反应性：Human，Mouse，Rat

经测试应用：WB，IHC，IF/ICC

预测分子量：31kDa

免疫原：Synthetic peptide within C-terminal human IL1 beta.

纯化方式：Protein A affinity purified.

形式：Liquid

浓度：WB：1:100-1000     IHC：1:10-100    IF/ICC：1:10-100

存储液：PBS containing 0.02% sodium azide.

别名：Catabolin, IL 1, IL 1 beta, IL1 BETA, IL-1 beta, IL1B, IL-1B, IL1beta, IL1F2, Interleukin 1 beta, interleukin 1, beta，IL-1β

储存温度：-20℃,避免污染

Western blot analysis of IL-1 beta on different lysates with Rabbit anti-IL-1 beta antibody at 1/5,000 dilution.

Lane 1: THP-1 whole cell lysate
Lane 2: THP-1 treated with 80nM TPA overnight then treated with 100ng/mL LPS for 6 hours and 300ng/mL BFA for 3 hours whole cell lysate
Lysates/proteins at 20 µg/Lane.

Predicted band size: 31 kDa
Observed band size: 34 kDa

Exposure time: 10 seconds; ECL: PC-30002;

Immunohistochemical analysis of paraffin-embedded human kidney tissue using anti-IL-1 beta antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (1/50) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.