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Mouse Monoclonal Anti-HERC5 Magnetic Beads

目录号: PC-58035

E3 ISG15--protein ligase HERC5 (HERC5) is a member of the HERC family of ubiquitin ligases and functions as an interferon-induced E3 protein ligase that mediates ISGylation of target proteins. It is a multidomain protein with a HECT domain and five RCC1 repeats. HERC5 localizes to the cytoplasm and perinuclear region and acts as a modulator of the antiviral immune response. It broadly modifies newly synthesized proteins in a cotranslational manner, with viral proteins being primary targets in interferon-stimulated cells. HERC5-mediated ISGylation has been shown to interfere with the function of key viral structural proteins, such as ebolavirus VP40 or HIV-1 GAG, and attenuate the virulence of influenza A virus by ISGylating the NS1 protein. Dysregulation of HERC5 has been associated with influenza. Its ability to promote the production of cytokines that facilitate bacterial clearance highlights its importance in the immune response beyond viral infections.

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规格 价格 库存
1ml 4000.00 现货
5ml 8000.00 现货

基本信息

产品应用:免疫沉淀(IP)

宿主:小鼠/IgG1

产品属性:

珠子直径:0.5 μm

反应种属:人

同源性:Highest antigen sequence identity to the following orthologs: Mouse - 61%, Rat - 41%, Pig - 76%, Cynomolgus monkey - 96%

稀释比:50ul/Test

免疫原序列:RESEFALRPTFDLTVRRNHLIEDVLNQLSQFENEDLRKELWVSFSGEIGYDLGGVKKEFFYCLFAEMIQPEYGMFMYPEGASCMWFPVKPKFEKKRYFFFGVLCGLSLFNCNVANLPFPLALFKKLLDQMPSLEDLKELSPDLGKNLQTLLDDEGDNFEEVFYIHFNVHWDRNDTNLIPNGSSITVNQTNKRDYVSKYINYIFNDSVKAVYEEFRRGFYKMCDEDIIKLFHPEELKDVIVGNTDYDWKTFEKNARYEPGYNSSHPTIVMFWKAFHKLTLEEKKKFLVFLTGTDRLQMKDLNNMKITFCCPESWNERDPIRALTCFSVLFLPKYSTMETVEEALQEAINNN

储存液:Supplied as solution in phosphate buffered saline containing 0.01% BSA and 0.02% sodium azide

 

 

50 μL of Mouse Monoclonal Anti-HERC5 Magnetic Beads were pre-washed with cell lysis buffer for 3 times. The beads were then incubated with HEK-293 lysate containing 200 µg of total protein for immunoprecipitation. The supernatant was discarded and beads were washed for 6 times. The beads were boiled with denaturing protein loading buffer for 10 min. The mixture was run on 6-18% SDS-PAGE and blotted onto nitrocellulose membrane. Mouse Monoclonal HERC5 antibody was used as the primary antibody and peroxidase conjugated goat anti-mouse IgG was used as the secondary antibody. Anti-KLH Magnetic Beads serves as negative control.

Lane 1: HEK-293 lysate

Lane 2: HERC5 immunoprecipitated from HEK-293 lysate by Mouse Monoclonal Anti-HERC5 Magnetic Beads

Lane 3: The same as Lane 2 but Anti-KLH Magnetic Beads were used as negative control.

Result: Mouse Monoclonal Anti-HERC5 Magnetic Beads can immunoprecipitate HERC5.

存储温度 -20℃

相关说明文档

储备液配置

注:请根据所选浓度进行配置储备液,配置好储备液后进行分装低温保存,避免反复冻融!PCM提供多种溶剂,如:DMSO(PC-57930);玉米油(PC-86094);棉籽油(PC-30875)等

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