名称：兔多克隆 TLR9 抗体

描述：The protein encoded by this gene is a member of the Toll-like receptor (TLR) family which plays a fundamental role in pathogen recognition and activation of innate immunity. TLRs are highly conserved from Drosophila to humans and share structural and functional similarities. They recognize pathogen-associated molecular patterns (PAMPs) that are expressed on infectious agents, and mediate the production of cytokines necessary for the development of effective immunity. The various TLRs exhibit different patterns of expression. This gene is preferentially expressed in immune cell rich tissues, such as spleen, lymph node, bone marrow and peripheral blood leukocytes. Studies in mice and human indicate that this receptor mediates cellular response to unmethylated CpG dinucleotides in bacterial DNA to mount an innate immune response.

克隆：多克隆

浓度： 1mg/ml

同种型：IgG

宿主：Rabbit

种属反应性：Human，Mouse，Rat

经测试应用：WB，IHC

预测分子量：135kDa

免疫原：A synthetic peptide of human TLR9(NP_059138.1).

纯化方式：The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

形式：Liquid

浓度：WB：1:500-1000     IHC：1:50-200    

别称：TLR9;CD289

存储液：PBS with 0.02% sodium azide,50% glycerol,pH7.3.

储存温度：-20℃,避免污染

Western blot analysis of extracts of Raji cells, using TLR9 antibody at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.

Immunohistochemistry of paraffin-embedded rat spleen using TLR9 Rabbit pAb at dilution of 1:100 .Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.

Immunohistochemistry of paraffin-embedded human liver using TLR9 Rabbit pAb at dilution of 1:100 .Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.