名称：兔多克隆 RIP3 抗体

描述：Certain serine/threonine protein kinases, such as ASK1, RIP, DAP, and ZIP kinases, are mediators of apoptosis. Receptor interacting proteins including RIP and RIP2/RICK mediate apoptosis induced by TNFR1 and Fas, two prototype members in the death receptor family. A novel member in the RIP kinase family was recently identified and designated RIP3. RIP3 contains N-terminal kinase domain but, unlike RIP or RIP2, lacks the C-terminal death or CARD domain. RIP3 binds to RIP and TNFR1, mediates TNFR1 induced apoptosis, and attenuates RIP and TNFR1 induced NF-κB activation. Overexpression of RIP3 induces apoptosis and NF-κB activation. The messenger RNA of RIP3 is expressed in a subset of adult tissues.

克隆：多克隆

浓度： 1mg/ml

同种型：IgG

宿主：Rabbit

种属反应性：Mouse，Rat

经测试应用：WB，IHC-P，IP

预测分子量：57kDa

免疫原：Raised against a peptide corresponding to amino acids 473 to 486 of murine RIP3.

纯化方式：Affinity chromatography purified via peptide column

形式：Liquid

浓度：WB：1:500-3000     IHC：1:100-1000     IP：1:20-200

存储液：PBS containing 0.02% sodium azide.

储存温度：-20℃,避免污染

Western Blot Validation in Mouse Cell lines
Loading: 15 μg of lysates per lane.

Antibodies: RIP3, (0.5 μg/mL), 1h incubation at RT in 5% NFDM/TBST.

Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution.

Western Blot Validation in C2C12 Cells Mouse
Loading: 15 μg of lysates per lane.

Antibodies: RIP3, 1h incubation at RT in 5% NFDM/TBST.

Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution.

Lane 1:  0.1 μg/mL in the presence of peptide blockingLane 2: 0.1 μg/mLLane 3: 0.2 μg/mLLane 4: 0.5 μg/mL

Western Blot Validation in Rat Thymus
Loading: 15 µg of lysates per lane.

Antibodies: RIP3, 0.5 μg/mL, 1h incubation at RT in 5% NFDM/TBST.

Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution.

 Immunohistochemistry Validation of RIP3 in Mouse Lung

Immunohistochemistry Validation of RIP3 in Rat Thymus
Immunohistochemical analysis of paraffin-embedded rat thymus tissue using anti-RIP3 antibody at 1 μg/ml. Tissue was fixed with formaldehyde and blocked with 10% serum for 1 h at RT; antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody overnight at 4°C. A goat anti-rabbit IgG H&L (HRP) at 1/250 was used as secondary. Counter stained with Hematoxylin.