Rabbit Monoclonal DUSP1 antibody
The expression of DUSP1 gene is induced in human skin fibroblasts by oxidative/heat stress and growth factors. It specifies a protein with structural features similar to members of the non-receptor-type protein-tyrosine phosphatase family, and which has significant amino-acid sequence similarity to a Tyr/Ser-protein phosphatase encoded by the late gene H1 of vaccinia virus. The bacterially expressed and purified DUSP1 protein has intrinsic phosphatase activity, and specifically inactivates mitogen-activated protein (MAP) kinase in vitro by the concomitant dephosphorylation of both its phosphothreonine and phosphotyrosine residues. Furthermore, it suppresses the activation of MAP kinase by oncogenic ras in extracts of Xenopus oocytes.
| 规格 | 价格 | 库存 |
|---|---|---|
| 50ul | 1800.00 | 现货 |
| 100ul | 3200.00 | 现货 |
基本信息
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名称：兔单克隆 DUSP1 抗体 描述：The expression of DUSP1 gene is induced in human skin fibroblasts by oxidative/heat stress and growth factors. It specifies a protein with structural features similar to members of the non-receptor-type protein-tyrosine phosphatase family, and which has significant amino-acid sequence similarity to a Tyr/Ser-protein phosphatase encoded by the late gene H1 of vaccinia virus. The bacterially expressed and purified DUSP1 protein has intrinsic phosphatase activity, and specifically inactivates mitogen-activated protein (MAP) kinase in vitro by the concomitant dephosphorylation of both its phosphothreonine and phosphotyrosine residues. Furthermore, it suppresses the activation of MAP kinase by oncogenic ras in extracts of Xenopus oocytes. 克隆：单克隆 纯化方式：Protein A affinity purified. 宿主：Rabbit/IgG 浓度：1mg/ml 种属反应性：Human，Mouse，Rat 经测试应用：WB，IHC-P/IHC-Fr，FC，IF/ICC，IP 预测分子量：39kDa 阳性对照：Jurkat cell lysate, SK-Br-3 cell lysate, NIH/3T3 cell lysate, mouse liver tissue lysate, mouse kidney tissue lysate, rat liver tissue lysate, rat kidney tissue lysate, NIH/3T3, C6, human lung cancer tissue, mouse lung tissue, rat lung tissue. 抗原：Synthetic peptide within Human DUSP1 aa 112-156 / 367. 应用： WB：1:500-2000 IHC-P/IHC-Fr：1:100-500 ICC/IF：1:50-200 FC：1:500-1000 IP：1-2μg/sample 存储形式：Liquid 别名：CL100；Dual specificity protein phosphatase 1；HVH1；MKP1；PTPN10 亚细胞定位：Nucleus. 存储液：1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. 储存温度：-20℃
Western blot analysis of DUSP1 on different lysates with Rabbit Monoclonal DUSP1 antibody at 1/2,000 dilution. Lysates/proteins at 20 µg/Lane. Exposure time: 5 minutes 10 seconds;
Immunocytochemistry analysis of NIH/3T3 cells labeling DUSP1 with Rabbit Monoclonal DUSP1 antibody at 1/100 dilution.
Immunohistochemical analysis of paraffin-embedded human lung cancer tissue with Rabbit Monoclonal DUSP1 antibody at 1/1,000 dilution.
Lane 3: Rabbit IgG instead of Rabbit Monoclonal DUSP1 antibody in SK-Br-3 cell lysateBlocking/Dilution buffer: 5% NFDM/TBST |
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| 存储温度 | -20℃ |
DUSP1 was immunoprecipitated from 0.2 mg SK-Br-3 cell lysate with Rabbit Monoclonal DUSP1 antibody at 2 µg/10 µl beads. Western blot was performed from the immunoprecipitate using Rabbit Monoclonal DUSP1 antibody at 1/1,000 dilution. Anti-Rabbit IgG for IP Nano-secondary antibody at 1/5,000 dilution was used for 1 hour at room temperature.Lane 1: SK-Br-3 cell lysate (input)相关说明文档
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