名称：小鼠单克隆 NCR1/NKp46 抗体

描述：NKp46（也称为 NCR1 或 CD335）是由 NCR1 基因编码的一种 46 kDa 的糖蛋白，属于免疫球蛋白（Ig）超家族。 它由两个 C2 型的胞外 Ig 样结构域组成，并通过其带正电荷的跨膜结构域与信号适配蛋白相互作用。 NKp46 是人类 NK 细胞的主要激活受体，在哺乳动物中高度保守，广泛表达于 NK 细胞、ILC1、NCR+ ILC3 和少量 T 细胞中。

宿主：Mouse IgG2a

别名：Natural cytotoxicity triggering receptor 1; Lymphocyte antigen 94 homolog; NK cell-activating receptor; Natural killer cell p46-related protein; NK-p46; NKp46; hNKp46; CD antigen CD335

种属反应性：Human

经测试应用：IHC-P

抗原序列：IWAEPHFMVPKEKQVTICCQGNYGAVEYQLHFEGSLFAVDRPKPPERINKVKFYIPDMNSRMAGQYSCIYRVGELWSEPSNLLDLVVTEM

浓度： IHC-P：1:500-1000

存储液：PBS with 50% glycerol, 0.5% BSA and 0.09% sodium azide

储存温度：-20℃

IHC-P was performed using sections of the formalin-fixed paraffin-embedded tonsil tissue. Antigen was retrieved through addition of boiling Tris/EDTA buffer pH 9 in a pressure cooker for 3 min. Endogenous peroxidase activity was quenched by incubating the sections with 3% H2O2 for 30 min at room temperature. The sections were then incubated with NCR1 antibody at room temperature for 1 h. Poly-peroxidase conjugated goat anti-mouse IgG was used as the secondary antibody. Diaminobenzidine was used as the chromogen. The section was counterstained with hematoxylin. A tissue section incubated with phosphate-buffered saline followed by incubation with the secondary antibody was used as the background control.

Result: A subset of cells in tonsil tissue are positively stained at the cell membrane.