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Rabbit Monoclonal NDRG1 antibody

目录号: PC-53924

Protein NDRG1 is a protein that in humans is encoded by the NDRG1 gene. This gene is a member of the N-myc downregulated gene family which belongs to the alpha/beta hydrolase superfamily. The protein encoded by this gene is a cytoplasmic protein involved in stress responses, hormone responses, cell growth, and differentiation[citation needed]. Mutations in this gene have been reported to be causative the autosomal-recessive version of Charcot-Marie-Tooth disease known as CMT4D. It has been reported that NDRG1 localizes to the endosomes and is a Rab4a effector involved in vesicular recycling. 

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规格 价格 库存
50ul 1800.00 现货
100ul 3200.00 现货

基本信息

名称:兔单克隆 NDRG 1 抗体

描述:Protein NDRG1 is a protein that in humans is encoded by the NDRG1 gene. This gene is a member of the N-myc downregulated gene family which belongs to the alpha/beta hydrolase superfamily. The protein encoded by this gene is a cytoplasmic protein involved in stress responses, hormone responses, cell growth, and differentiation[citation needed]. Mutations in this gene have been reported to be causative the autosomal-recessive version of Charcot-Marie-Tooth disease known as CMT4D. It has been reported that NDRG1 localizes to the endosomes and is a Rab4a effector involved in vesicular recycling. 

克隆:单克隆

纯化方式:Protein A affinity purified.

宿主:Rabbit/IgG

浓度:1mg/ml

种属反应性:Human,Mouse,Rat

经测试应用:WB,IHC-P/IHC-Fr,IP,IF/ICC

预测分子量:43kDa

阳性对照:PC-3 cell lysate, SW480 cell lysate, RPMI 8226 cell lysate, RAW264.7 cell lysate, Mouse eyeball tissue lysate, Rat eyeball tissue lysate, MCF-7, A431, human prostate tissue, mouse kidney tissue, human colon carcinoma tissue.

抗原:Synthetic peptide within Human NDRG1 aa 345-394 / 394.

应用:  WB:1:500-2000        IHC-P/IHC-Fr:1:50-200      ICC/IF:1:50-200    IP:1:50 

存储形式:Liquid

别名:42 kDa antibody

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亚细胞定位:Cytoplasm. Nucleus. Cell membrane.

存储液:1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

储存温度:-20℃

 

 

Western blot analysis of NDRG1 on different lysates with Rabbit anti-NDRG1 antibody at 1/1,000 dilution.

 

 

 

 

 

 

 

 

 

ICC/IF staining of NDRG1 in MCF-7 cells (green). Formalin fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 10 minutes at room temperature and blocked with 1% Blocker BSA for 15 minutes at room temperature. Cells were probed with the primary antibody (1/50) for 1 hour at room temperature, washed with PBS. Alexa Fluor®488 Goat anti-Rabbit IgG was used as the secondary antibody at 1/1,000 dilution. The nuclear counter stain is DAPI (blue).

 

 

 

 

 

 

 

Immunohistochemical analysis of paraffin-embedded human prostate tissue with Rabbit anti-NDRG1 antibody at 1/1,000 dilution.

存储温度 -20℃

相关说明文档

储备液配置

注:请根据所选浓度进行配置储备液,配置好储备液后进行分装低温保存,避免反复冻融!PCM提供多种溶剂,如:DMSO(PC-57930);玉米油(PC-86094);棉籽油(PC-30875)等

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